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9812
KinomeView® Profiling Kit
Primary Antibodies
PTMScan

KinomeView® Profiling Kit #9812

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Citations (1)
KinomeView® Profiling Kit: Image 1
Western blot analysis of extracts from HeLa cells, untreated or treated with Calyculin A #9902 (10 nM, 30 min) or Staurosporine #9953 (1 μM, 3 hr), using Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb #8738.
KinomeView® Profiling Kit: Image 2
Western blot analysis of extracts from COS-7 cells, synchronized in G1/S, G2/M, and G0 phase of the cell cycle, using Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb #9477.
KinomeView® Profiling Kit: Image 3
Phospho-Tyr antibody reacts broadly with Tyr-phosphorylated proteins. HeLa cells were treated by different conditions, an induction in Tyr phosphorylation is observed upon stimulation of EGF receptor by treatment with EGF; EGFR mediated phosphorylation is inhibited by the tyrosine kinase inhibitor gefitinib.
KinomeView® Profiling Kit: Image 4
Phospho-MAPK/CDK Substrate antibody has broad reactivity with proteins phosphorylated on Ser residues with proximal Pro residues at the -2 and +1 positions. CMGC family kinases including MAPKs and CDKs phosphorylate substrates containing this motif. Changes in phosphorylation of this motif are detected with nocodazole mediated mitotic arrest, as well as MEK/ERK signaling inhibition by U0126 in HeLa cells.
KinomeView® Profiling Kit: Image 5
Western blot analysis of extracts from various cell types, untreated or treated as indicated, using KinomeView Profiling Kit component antibodies. Select abbreviations: TBB, CKII inhibitor 4,5,6,7-tetrabromobenzotriazole; PDBu, phorbol 12,13-dibutyrate.
To Purchase # 9812
Cat. # Size Qty. Price
9812S
1 Kit  (16 x 40 microliters) $ 1,545

Products Included Motif Quantity Isotype
Phospho-Akt Substrate (110B7E) Rabbit mAb #9614 RXX(S*/T*) 40 µl Rabbit IgG
Phospho-Akt Substrate (23C8D2) Rabbit mAb #10001 RXRXX(S*/T*) 40 µl Rabbit IgG
Phospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb #5759 (L/M)XRXX(S*/T*), RXX(S*/T*) 40 µl Rabbit IgG
Phospho-(Ser) ATM/ATR Substrate (D23H2/D69H5) Rabbit mAb #9607 S*Q 40 µl Rabbit IgG
Phospho-(Ser/Thr) ATM/ATR Substrate (S*/T*QG) (P-S/T2-100) Rabbit mAb #6966 (S*/T*)QG, (S*/T*)Q 40 µl Rabbit IgG
Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb #9477 (K/H)S*P 40 µl Rabbit IgG
Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb #8738 (S*/T*)DXE 40 µl Rabbit IgG
Phospho-MAPK/CDK Substrates (34B2) Rabbit mAb #2325 PXS*P, S*PX(K/R) 40 µl Rabbit IgG
Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb #9634 (F/K)XX(F/Y)(S*/T*)(F/Y) 40 µl Mouse IgG2a
Phospho-PKA Substrate (100G7E) Rabbit mAb #9624 (K/R)(K/R)X(S*/T*) 40 µl Rabbit IgG
Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb #6967 (K/R)XS*X(K/R) 40 µl Rabbit IgG
Phospho-(Thr) PLK Binding Motif (D73F6) Rabbit mAb #5243 ST*P 40 µl Rabbit IgG
Phospho-Thr-Pro Motif Antibody #3003 † T*P, T*PP 40 µl Rabbit IgG
Phospho-Thr-Pro-Glu (C32G12) Rabbit mAb #3004 T*PE, T*P 40 µl Rabbit IgG
Phospho-Thr-X-Arg Motif Antibody #2351 T*X(K/R) 40 µl Rabbit IgG
Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb #8954 Y* 40 µl Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl Goat
Anti-mouse IgG, HRP-linked Antibody #7076 100 µl Horse

Product Description

The KinomeView® Profiling Kit provides a set of Phospho-Motif Antibodies that cover a large portion of the kinome and react broadly with serine, threonine, and tyrosine phosphorylation mediated by diverse kinase families throughout the kinome. This kit will provide researchers with an immunoblotting strategy to dissect the complexity of the phosphoproteome and determine the kinase families involved in the regulation of diverse physiological processes. By using the provided Phospho-motif Antibodies, the investigator can assess global changes in protein phosphorylation by western analysis across a range of experimental samples and conditions.

Specificity / Sensitivity

Each antibody recognizes endogenous levels of the respective target motif. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Background

Cell Signaling Technology (CST) has developed Phospho-motif Antibodies that have broad immunoreactivity for serine, threonine and tyrosine phosphorylation mediated by both known and unknown kinases. Using these Motif Antibodies for western blotting provides a Kinome-wide view of cellular phosphorylation.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.