NK1.1/CD161 (E6Y9G) Rabbit mAb (BSA and Azide Free) (#24395) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

IHC-Bond, IHC-P, IF-F, FC-FP

REACTIVITY:

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Rabbit IgG

UniProt ID:

#P27814

Entrez-Gene Id:

17059

Product Information

Product Usage Information

This product is the carrier free version of product #39197. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #39197.

Storage

Store at -20°C. emThis product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles./em A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

NK1.1/CD161 (E6Y9G) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD161c protein from C57BL/6, SJL, and NZW mice, but not from BALB/c and 129S6 mice. Mice from other strains have not been tested. This antibody is predicted to cross-react with CD161b and may cross-react with CD161a protein in some mouse strains. Non-specific staining was observed in mouse testis by immunohistochemistry.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse NK1.1/CD161 protein.

Background

CD161/KLRB1 (Killer cell lectin-like receptor subfamily B member 1, also known as CLEC5B and NKR-P1A) is a type II transmembrane protein that is expressed on the majority of Natural Killer (NK) cells, NK T cells, and some T lymphocytes (1). CD161/KLRB1 is also expressed on Th17 cells, promotes their generation, and modulates their function (2). Engagement with its ligand lectin-like transcript 1 (LLT1) inhibits NK cell function, while LLT1 and CD161/KLRB1 interaction in the presence of a TCR signal enhances IFN-gamma production by T cells (3,4). There are several different CD161 isoforms in rodents and some function as activating receptors as well (5,6).

There is a family of Klrb1 genes in rodents (7). PK136 antibody recognizes a specific epitope on mouse KLRB1b/c, also referred to as NK1.1, and is commonly used for detection of mouse NK cells in certain mouse strains (CE, B6, NZB, C58, Ma/My, ST, SJL, FVB). However, the epitope is absent in other mouse strains (BALB/c, AKR, CBA, C3H, DBA, 129) (8).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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