WB
H M R
Endogenous
58
Rabbit
#P52292
3838
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu66 of human KPNA2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Importins belong to the karyopherin family of nuclear transport proteins and are divided into two subgroups: importin alpha and importin beta. Importins function mainly in the import and export of nuclear proteins (1,2). KPNA2 (karyopherin alpha 2), a member of the importin alpha family, contains an N-terminal importin beta binding (IBB) motif followed by a hydrophobic region consisting of 10 armadillo repeats that function in binding to the nuclear localization signal (NLS) sites of cargo proteins (3-5). A trimeric complex (importin beta/KPNA2/cargo protein) forms, translocates into the nucleus, and then dissociates upon binding of RanGTP to importin beta. The dissociated importin alpha is recycled back to the cytoplasm with the help of export factor CAS (6,7). KPNA2 can differentially regulate target localization by binding to different cargo proteins, either actively transporting them to the nucleus (such as oct3/4) or retaining them in the cytoplasm by formation of incompetent complexes (such as oct6/brn2) (8). Research studies indicate that KPNA2 promotes cell proliferation and tumorigenesis. Research studies have also shown that up-regulation of KPNA2 is associated with cancer progression. Therefore, it has become a focus of biomarker research (9-13).
- Chook, Y.M. and Blobel, G. (2001) Curr Opin Struct Biol 11, 703-15.
- Pemberton, L.F. and Paschal, B.M. (2005) Traffic 6, 187-98.
- Kelley, J.B. et al. (2010) BMC Cell Biol 11, 63.
- Weis, K. et al. (1995) Science 268, 1049-53.
- Fontes, M.R. et al. (2003) J Biol Chem 278, 27981-7.
- Görlich, D. (1998) EMBO J 17, 2721-7.
- Goldfarb, D.S. et al. (2004) Trends Cell Biol 14, 505-14.
- Yasuhara, N. et al. (2013) Dev Cell 26, 123-35.
- Huang, L. et al. (2013) Cell Death Dis 4, e745.
- Grupp, K. et al. (2014) Mod Pathol 27, 96-106.
- Altan, B. et al. (2013) Carcinogenesis 34, 2314-21.
- Rachidi, S.M. et al. (2013) PLoS One 8, e57911.
- He, L. et al. (2012) PLoS One 7, e42992.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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