WB, IF-IC
H
Endogenous
105, 150
Rabbit IgG
#Q13753
3918
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:1000 - 1:2000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His747 of human LAMC2 protein.
Background
Laminins are a family of proteins important for maintaining cellular basement membrane (BM) structure and function (1). Laminins exist as heterotrimers of α (LAMA), β (LAMB), and γ (LAMC) subunits. LAMC2 is the γ subunit of laminin-5 (LN-332 or LN5), which is a triplex composed of laminin α3, β3, and γ2 chains. LN-332 is one of the main isoforms of laminin associated with malignant transformation, proliferation, adhesion, migration, invasion, and metastasis (2). Upregulation of LAMC2 is a prognostic marker for the progression of various cancer types, including colorectal carcinoma, urinary bladder carcinoma, pancreatic ductal adenocarcinoma, and lung carcinoma (3-6). Under physiological conditions, LN-332 locates at the dermal-epidermal junction, provides skin integrity and resistance to external mechanical forces, and promotes adhesion and migration of epithelial cells (7,8). Mutation or deletion of the LAMC2 gene causes Herlitz junctional epidermolysis bullosa, an inherited disease causing severe skin blistering (9,10). The biological activity of LN-332 can be regulated by LAMC2 proteolytic processing through MT1-MMP (MMP14) (11). LN-332 containing full-length LAMC2 (150 kDa) functions to promote adhesion, while LN-332 containing processed LAMC2 (105 kDa) promotes migration (12).
- Yurchenco, P.D. (2011) Cold Spring Harb Perspect Biol 3, a004911. doi: 10.1101/cshperspect.a004911.
- Meireles Da Costa, N. et al. (2021) Cancers (Basel) 13, 1890. doi: 10.3390/cancers13081890.
- Nilsson, P.J. et al. (2005) Ann Oncol 16, 893-8.
- Kiyoshima, K. et al. (2005) Hum Pathol 36, 522-30.
- Okada, Y. et al. (2021) Carcinogenesis 42, 546-556.
- Liu, M. et al. (2021) Ann Transl Med 9, 1392.
- Salo, S. et al. (1999) Matrix Biol 18, 197-210.
- Kiritsi, D. et al. (2013) Cell Adh Migr 7, 135-41.
- Takizawa, Y. et al. (1998) J Invest Dermatol 111, 1233-4.
- Meng, X. et al. (2003) J Invest Dermatol 121, 720-31.
- Koshikawa, N. et al. (2005) J Biol Chem 280, 88-93.
- Ogawa, T. et al. (2004) J Cell Biochem 92, 701-14.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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