WB, IHC-P
H
Endogenous
22
Rabbit IgG
#P80188
3934
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:3000 - 1:12000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala73 of human LCN2 protein.
Background
Lipocalin 2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), belongs to the lipocalin family. Proteins in this family exhibit considerable sequence diversity, but share a highly conserved structure composed of an eight-stranded antiparallel beta-barrel that encloses an internal ligand-binding site (1,2). LCN2 is considered as an iron transporter. LCN2 can bind bacterial siderophores and inhibits bacterial growth by iron depletion (3). It can also bind endogenous compounds like catechol in complex with iron (4). LCN2 was originally identified in neutrophils, and its expression was induced in many other tissues like kidney and liver in response to various pathologic states, such as infection, ischemia, and acute kidney injury. LCN2 is considered a biomarker for conditions like ischemic stroke, acute kidney injury, inflammatory and metabolic diseases (5-7).
- Flower, D.R. (1996) Biochem J 318 ( Pt 1), 1-14.
- Grzyb, J. et al. (2006) J Plant Physiol 163, 895-915.
- Goetz, D.H. et al. (2002) Mol Cell 10, 1033-43.
- Bao, G. et al. (2010) Nat Chem Biol 6, 602-9.
- Haase-Fielitz, A. et al. (2014) Ann Clin Biochem 51, 335-51.
- Abella, V. et al. (2015) Biomarkers 20, 565-71.
- Hochmeister, S. et al. (2016) PLoS One 11, e0154797.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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