WB
H
Endogenous
37
Rabbit IgG
#P07195
3945
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala13 of human LDHB protein.
Background
Lactate dehydrogenase (LDH) catalyzes the reversible conversion between pyruvate and lactate. LDH is a tetramer composed of various combinations of LDHA subunit and LDHB subunit to form five different isozymes. LDHA has a higher affinity for pyruvate and preferentially catalyzes the conversion of pyruvate to lactate. LDHA levels are upregulated in many cancers. On the other hand, LDHB has a higher affinity for lactate and preferentially catalyzes the conversion of lactate to pyruvate, enabling cells to use lactate as a nutrient (1-3). Studies show that LDHA/LDHB deficiency suppresses glycolysis and ATP production, inhibiting STING signaling and antitumor immune responses mediated by dendritic cells (4). In addition, acetylation of LDHB inhibits its activity, reduces hepatic lactate clearance, and promotes the progression of non-alcoholic fatty liver disease (NAFLD) (5).
- Doherty, J.R. and Cleveland, J.L. (2013) J Clin Invest 123, 3685-92.
- Hong, S.M. et al. (2019) J Biol Chem 294, 7810-7820.
- Urbańska, K. and Orzechowski, A. (2019) Int J Mol Sci 20, 2085. doi: 10.3390/ijms20092085.
- Hu, Z. et al. (2023) J Clin Invest 133, e166031. doi: 10.1172/JCI166031.
- Wang, T. et al. (2021) J Hepatol 74, 1038-1052.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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