WB, IP, IF-IC, FC-FP
H
Endogenous
45-70
Rabbit IgG
#P36941
4055
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:600 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg428 of human LTβR protein.
Background
Lymphotoxin β receptor (LTβR), also known as tumor necrosis factor receptor superfamily member 3 (TNFRSF3) and tumor necrosis factor receptor type III (TNF-RIII), is a type I membrane receptor expressed by stromal cells, including endothelial, mesenchymal, and epithelial cells, and by myeloid cells, such as dendritic cells and macrophages (1-4). When lymphotoxin α (LTα) is co-expressed with lymphotoxin β (LTβ) that possesses a transmembrane domain, it forms a cell surface-bound heterotrimer (LTα1β2) that exclusively binds to LTβR (5,6). The LTα1β2/LTβR pathway is required for the normal development of lymph nodes and Peyer’s patches (7,8). Disruption of the LTα1β2/LTβR pathway results in impaired protection from viral and bacterial infections (9,10). LTβR is also a receptor for LIGHT/TNFSF14 (11,12). LTβR signaling through LIGHT/TNFSF14 regulates self-renewal and differentiation of hematopoietic and leukemic stem cells (13). In cancer cells, LTβR signaling via TRAF molecules promotes apoptosis (14-16). Agonistic antibodies to LTβR trigger cancer cell death and suppress tumor growth in vivo (17).
- Murphy, M. et al. (1998) Cell Death Differ 5, 497-505.
- Browning, J.L. and French, L.E. (2002) J Immunol 168, 5079-87.
- Browning, J.L. et al. (1997) J Immunol 159, 3288-98.
- Kabashima, K. et al. (2005) Immunity 22, 439-50.
- Androlewicz, M.J. et al. (1992) J Biol Chem 267, 2542-7.
- Browning, J.L. et al. (1993) Cell 72, 847-56.
- De Togni, P. et al. (1994) Science 264, 703-7.
- Fütterer, A. et al. (1998) Immunity 9, 59-70.
- Ehlers, S. et al. (2003) J Immunol 170, 5210-8.
- Wege, A.K. et al. (2014) Innate Immun 20, 461-70.
- Maeda, T. et al. (2018) J Immunol 201, 202-214.
- Schneider, K. et al. (2004) Immunol Rev 202, 49-66.
- Höpner, S.S. et al. (2021) Nat Commun 12, 1065.
- Browning, J.L. et al. (1996) J Exp Med 183, 867-78.
- Force, W.R. et al. (1997) J Biol Chem 272, 30835-40.
- Rooney, I.A. et al. (2000) J Biol Chem 275, 14307-15.
- Lukashev, M. et al. (2006) Cancer Res 66, 9617-24.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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