Revision 1
#37872
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W
Reactivity:
M
Sensitivity:
Endogenous
MW (kDa):
10
Source/Isotype:
Rabbit IgG
UniProt ID:
#P0CW02, #P0CW03
Entrez-Gene Id:
17067, 100041546
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity/Sensitivity
Source / Purification
Background
Ly-6 proteins have been widely used as differentiation markers on hematopoietic cells. The ability to isolate and express specific Ly-6 antibodies through hybridoma technology has allowed researchers to identify unique proteins (1). These proteins are expressed on subsets of immune cells at different stages of development, such as T cells, B cells, monocytes, granulocytes, and macrophages (1-5).
Ly-6C1 and Ly-6C2 are two distinct genes located adjacent to each other within the Ly-6 gene complex. They share >95% similarity in their genomic and protein sequences, and with a nearly identical structure, expression profile, and reactivity to antibodies, they are collectively known as Ly-6C. A propeptide is removed to form the mature Ly-6C protein, a 10 kDa GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is most frequently used to distinguish inflammatory states of monocyte populations in mice (6).
Background References
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting
Cross-Reactivity Key
M: Mouse
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Revision 1