WB, IP, IHC-P
M
Endogenous
60-70
Rabbit IgG
#Q8BHC0
114332
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Storage
For a carrier free (BSA and azide free) version of this product see product #93121.
Specificity / Sensitivity
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of mouse LYVE-1 protein.
Background
Lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1), also known as extracellular link domain-containing protein 1 (XLKD1), is a type I integral membrane glycoprotein. LYVE-1 is a CD44 homolog found primarily on lymphatic endothelial cells, but has also been described on hepatic blood sinusoidal endothelia, embryonic blood vessels, Kupffer cells, the islets of Langerhans, cortical neurons, and renal epithelium (1-3). LYVE-1 acts as a receptor for hyaluronan, and plays a role in hyaluronan transport and turnover (4-7). LYVE-1 also mediates the docking and entry of dendritic cells to lymphatic vessels through selective adhesion to hyaluronan in the leukocyte surface glycocalyx (8). Several studies have demonstrated the utility of LYVE-1 as a marker for lymphoid tissues and lymphangiogenesis (9,10).
- Mouta Carreira, C. et al. (2001) Cancer Res 61, 8079-84.
- Lee, H.W. et al. (2011) Cell Tissue Res 343, 429-44.
- Gordon, E.J. et al. (2008) Dev Dyn 237, 1901-9.
- Banerji, S. et al. (1999) J Cell Biol 144, 789-801.
- Lawrance, W. et al. (2016) J Biol Chem 291, 8014-30.
- Prevo, R. et al. (2001) J Biol Chem 276, 19420-30.
- Stanly, T.A. et al. (2020) J Biol Chem 295, 5036-5050.
- Johnson, L.A. et al. (2017) Nat Immunol 18, 762-770.
- Skobe, M. et al. (2001) Nat Med 7, 192-8.
- Beasley, N.J. et al. (2002) Cancer Res 62, 1315-20.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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