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73959
Matrix Remodeling Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Matrix Remodeling Antibody Sampler Kit #73959

Citations (3)

Simple Western™ analysis of lysates (1.0 mg/mL) from 3T3-L1 cells using MMP-2 (D2O4T) Rabbit mAb #87809. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Western blot analysis of concentrated, serum-free cultured medium from U-2 OS cells, untreated (-) or treated with TPA #4174 (200 nM, 48 hr; +), using MMP-9 (D6O3H) XP® Rabbit mAb.
Western blot analysis of extracts from various cell lines using MMP-3 (D7F5B) Rabbit mAb.
Western blot analysis of extracts from various cell lines using MT1-MMP (E3S5S) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of MT1-MMP protein in HCC1419 cells is consistent with the predicted expression pattern.
Western blot analysis of extracts from A-431, and NIH/3T3 cells, using TIMP3 (D74B10) Rabbit mAb.
Western blot analysis of extracts from HT-1080, A172, and COS-7 cells using TIMP2 (D18B7) Rabbit mAb.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of extracts from concentrated culture medium of IGROV-1 and HT-29 cell lines using MMP-7 Antibody.
Western blot analysis of extracts from U87, 3T3-L1 and C2C12 cells using MMP-2 (D4O4T) Rabbit mAb.
Western blot analysis of extracts from various cell lines using TIMP1 (D10E6) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using TIMP1 (D10E6) Rabbit mAb.
Western blot analysis of extracts and concentrated culture medium from U-2 OS cells, untreated (-) or treated with TPA #4174 (200 nM, 48 hr; +) using MMP-9 (D6O3H) XP® Rabbit mAb. MMP-9 is induced by TPA treatment as expected.
Immunoprecipitation of MMP-2 protein from U87 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MMP-2 (D2O4T) Rabbit mAb. Western blot analysis was performed using MMP-2 (D2O4T) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using TIMP1 (D10E6) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using MMP-9 (D6O3H) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human esophageal caricinoma using TIMP1 (D10E6) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using MMP-9 (D6O3H) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using TIMP1 (D10E6) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded U-2 OS cell pellets, untreated (left) or treated with TPA #4174 (right), using MMP-9 (D6O3H) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded normal human pancreas using TIMP1 (D10E6) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using MMP-9 (D6O3H) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemical analysis of paraffin-embedded normal human small intestine using TIMP1 (D10E6) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using TIMP1 (D10E6) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Flow cytometric analysis of U-2 OS cells, untreated (blue) or treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (200 nM, 24 hr; green) using MMP-9 (D6O3H) XP® Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Immunohistochemical analysis of paraffin-embedded U-87 MG cell pellet (left, positive) or Ramos cell pellet (right, negative) using TIMP1 (D10E6) Rabbit mAb.
To Purchase # 73959
Cat. # Size Qty. Price
73959T
1 Kit  (8 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
MMP-9 (D6O3H) XP® Rabbit mAb 13667 20 µl
  • WB
  • IHC
  • F
H 84, 92 Rabbit IgG
MMP-2 (D2O4T) Rabbit mAb 87809 20 µl
  • WB
  • IP
H M 64,72 Rabbit IgG
MMP-3 (D7F5B) Rabbit mAb 14351 20 µl
  • WB
H R 60 Rabbit IgG
TIMP1 (D10E6) Rabbit mAb 8946 20 µl
  • WB
  • IHC
H Mk 26 Rabbit IgG
TIMP2 (D18B7) Rabbit mAb 5738 20 µl
  • WB
H M Mk 22 Rabbit IgG
TIMP3 (D74B10) Rabbit mAb 5673 20 µl
  • WB
H M R 20, 25 Rabbit IgG
MMP-7 Antibody 71031 20 µl
  • WB
H 28 Rabbit 
MT1-MMP (E3S5S) Rabbit mAb 26424 20 µl
  • WB
H 62 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Rab Goat 

Product Description

The Matrix Remodeling Antibody Sampler Kit provides an economical means of detecting different MMPs and TIMPs using the specific corresponding antibodies. The kit contains enough antibody to perform at least two western blot experiments with each primary antibody.

Background

Matrix remodeling is mainly controlled by MMPs and TIMPs. The matrix metalloproteinase (MMP) family of proteases are a group of zinc-dependent enzymes that target extracellular proteins, including growth factors, cell surface receptors, adhesion molecules, matrix structural proteins, and other proteases (1, 2). Among the family members, MMP-2, MMP-3, MMP-7, MMP-9, and MMP14 (MT1-MMP) have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis, and apoptosis (3). MMP activity is regulated by mechanisms of both transcriptional control and post translational protein processing. Once synthesized, MMPs exist as latent proenzymes. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full-length protein (4). MMP activity can be inhibited through its binding to endogenously expressed TIMPs. TIMPs are members of the family of tissue inhibitors of matrix metalloproteinases that include TIMP1, TIMP2, TIMP3, and TIMP4. The main function of TIMPs is their inhibitory effect on MMPs. TIMPs irreversibly inactivate MMPs by direct binding MMPs and chelating their zinc cofactor at the catalytic site to inhibit the proteinase function (5,6).

Pathways

Explore pathways related to this product.

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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