WB, IP
H
Endogenous
13-15
Rabbit
#P13500
6347
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MCP-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Monocyte chemotactic protein-1 (MCP-1), also known as CCL2, monocyte chemotactic activating factor (MCAF) or glioma-derived chemotactic factor-2 (GDCF-2), is the product of the human JE gene and a member of the family of C-C (or β) chemokines (1-4). The predicted molecular weight of MCP-1 protein is 11-13 kDa, but it may migrate at 20-30 kDa due to glycosylation. MCP-1 is secreted by a variety of cell types in response to pro-inflammatory stimuli and was originally described for its chemotactic activity on monocytes. This activity has led to studies demonstrating its role in diseases characterized by monocyte infiltrates such as psoriasis (5), rheumatoid arthritis (6) and atherosclerosis (7). MCP-1 may also contribute to tumor progression and angiogenesis (8). Signaling by MCP-1 is mediated by the G-protein coupled receptor CCR2 (9).
- Matsushima, K. et al. (1989) J Exp Med 169, 1485-90.
- Furutani, Y. et al. (1989) Biochem Biophys Res Commun 159, 249-55.
- Robinson, E.A. et al. (1989) Proc Natl Acad Sci USA 86, 1850-4.
- Rollins, B.J. et al. (1988) Proc Natl Acad Sci USA 85, 3738-42.
- Gillitzer, R. et al. (1993) J Invest Dermatol 101, 127-31.
- Koch, A.E. et al. (1992) J Clin Invest 90, 772-9.
- Ylä-Herttuala, S. et al. (1991) Proc Natl Acad Sci USA 88, 5252-6.
- Salcedo, R. et al. (2000) Blood 96, 34-40.
- Charo, I.F. et al. (1994) Proc Natl Acad Sci USA 91, 2752-6.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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