WB
H
Endogenous
230
Rabbit IgG
#Q9UHV7
9969
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1592 of human MED13 protein.
Background
The mammalian Mediator complex is a multi-subunit protein complex that acts to relay signals from transcription factors to RNA polymerase II (Pol II) and the basal transcription machinery. In other words, Mediator acts as a centralized hub or integrator for transcriptional regulation (reviewed in 1,2). Multiple studies have shown that Mediator functions include epigenetic regulation, transcriptional elongation, transcription termination, mRNA processing, non-coding RNA activation, and super-enhancer formation. Through these functions, Mediator contributes to the regulation of many biological processes, including insulin signaling (3), NF-κB-dependent signaling (4), stem cell pluripotency and self-renewal (5,6), and proliferation of colon cancer cells (7,8). The Mediator complex consists of 31 protein subunits and can be divided into four distinct modules: the head, middle, tail, and CDK8 kinase module. The CDK8 kinase module contains the CDK8, cyclin C, MED12, and MED13 subunits and is thought to act as a molecular switch, phosphorylating the C-terminal domain (CTD) of Rpb1, the large subunit of Pol II, to inhibit Pol II recruitment and transcription initiation (9,10).
- Malik, S. and Roeder, R.G. (2005) Trends Biochem Sci 30, 256-63.
- Soutourina, J. (2018) Nat Rev Mol Cell Biol 19, 262-274.
- Wang, W. et al. (2009) Dev Cell 16, 764-71.
- van Essen, D. et al. (2009) PLoS Biol 7, e73.
- Tutter, A.V. et al. (2009) J Biol Chem 284, 3709-18.
- Varelas, X. et al. (2008) Nat Cell Biol 10, 837-48.
- Firestein, R. et al. (2008) Nature 455, 547-51.
- Morris, E.J. et al. (2008) Nature 455, 552-6.
- Knuesel, M.T. et al. (2009) Mol Cell Biol 29, 650-61.
- Knuesel, M.T. et al. (2009) Genes Dev 23, 439-51.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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