WB, IP, ChIP
H
Endogenous
70
Rabbit IgG
#O95402
9441
Product Information
Product Usage Information
For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Chromatin IP | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro120 of human MED26 protein.
Background
The mediator complex consists of about 25-30 proteins and is thought to facilitate transcription activation by acting as a molecular bridge between the RNA polymerase II (RNAPII) machinery and transcription factors (1). Mediator is recruited to target genes by transcription factors and plays an essential role in the recruitment and stabilization of the RNAPII transcription complex at promoters, as well as the activation of transcription post RNAPII recruitment (1-5). The mediator complex also plays an important role in creating ‘chromatin loops’ that occur as a result of interactions between the transcription factor bound at distal enhancers and RNAPII bound at the proximal promoter, and works to sustain proper chromatin architecture during active transcription (6-8).
Mediator complex subunit 26 (MED26) plays a critical role in the transition of RNAPII from the initiation to elongation phase of transcription. The amino-terminal domain of MED26 is required for recruitment of the ELL- and pTEFb-containing complexes to target genes, which facilitates transcription elongation (9).
- Taatjes, D.J. (2010) Trends Biochem Sci 35, 315-22.
- Kornberg, R.D. (2005) Trends Biochem Sci 30, 235-9.
- Malik, S. and Roeder, R.G. (2005) Trends Biochem Sci 30, 256-63.
- Malik, S. et al. (2002) Mol Cell Biol 22, 5626-37.
- Wang, G. et al. (2005) Mol Cell 17, 683-94.
- Kagey, M.H. et al. (2010) Nature 467, 430-5.
- Park, S.W. et al. (2005) Mol Cell 19, 643-53.
- Chen, Z. et al. (2011) EMBO J 30, 2405-19.
- Takahashi, H. et al. (2011) Cell 146, 92-104.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation ChIP: Chromatin IP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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