| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| MED1 Antibody | 51613 | 20 µl | 220 kDa | Rabbit |
| MED12 (D9K5J) Rabbit mAb | 14360 | 20 µl | 240 kDa | Rabbit IgG |
| MED26 (D4B1X) Rabbit mAb | 14950 | 20 µl | 70 kDa | Rabbit IgG |
| CDK8 (D6M3J) Rabbit mAb | 17395 | 20 µl | 53 kDa | Rabbit IgG |
| Cyclin C (E6V4Z) Rabbit mAb | 68179 | 20 µl | 30 kDa | Rabbit IgG |
| Rpb1 NTD (D8L4Y) Rabbit mAb | 14958 | 20 µl | 250 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
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Description
The Mediator Complex Antibody Sampler Kit provides an economical means of evaluating total levels of Mediator Complex proteins. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Storage
Background
The mammalian Mediator complex is a multi-subunit protein complex that acts to relay signals from transcription factors to RNA polymerase II (Pol II) and the basal transcription machinery. In other words, Mediator acts as a centralized hub or integrator for transcriptional regulation (reviewed in 1,2). Multiple studies have shown that Mediator functions include epigenetic regulation, transcriptional elongation, transcription termination, mRNA processing, non-coding RNA activation, and super-enhancer formation. Through these functions, Mediator contributes to the regulation of many biological processes, including insulin signaling (3), NF-κB-dependent signaling (4), stem cell pluripotency and self-renewal (5,6), and proliferation of colon cancer cells (7,8). The Mediator complex consists of 31 protein subunits and can be divided into four distinct modules: the head, middle, tail, and CDK8 kinase module. The CDK8 kinase module contains the CDK8, cyclin C, MED12, and MED13 subunits and is thought to act as a molecular switch, phosphorylating the C-terminal domain (CTD) of Rpb1, the large subunit of Pol II, to inhibit Pol II recruitment and transcription initiation (9,10).
- Malik, S. and Roeder, R.G. (2005) Trends Biochem Sci 30, 256-63.
- Soutourina, J. (2018) Nat Rev Mol Cell Biol 19, 262-274.
- Wang, W. et al. (2009) Dev Cell 16, 764-71.
- van Essen, D. et al. (2009) PLoS Biol 7, e73.
- Tutter, A.V. et al. (2009) J Biol Chem 284, 3709-18.
- Varelas, X. et al. (2008) Nat Cell Biol 10, 837-48.
- Firestein, R. et al. (2008) Nature 455, 547-51.
- Morris, E.J. et al. (2008) Nature 455, 552-6.
- Knuesel, M.T. et al. (2009) Mol Cell Biol 29, 650-61.
- Knuesel, M.T. et al. (2009) Genes Dev 23, 439-51.
Background References
Trademarks and Patents
Limited Uses
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