WB, IP
H
Endogenous
78
Rabbit
#Q9Y2U5
10746
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly292 of human MEKK2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Mitogen-activated protein kinase kinase kinase 2 (MEKK2/MAP3K2) belongs to the MAP3K family of Ser/Thr kinases. Research studies have demonstrated that MEKK2 plays a pivotal role in transducing mitogenic signals emanating from EGFR and FGF2R to JNK and ERK5 signaling cascades (1,2). Post-translationally, MEKK2 is regulated through multiple mechanisms, including dimerization (3,4), ubiquitination (5,6), phosphorylation (7), and methylation (8). Research studies implicate dysregulation of MEKK2 signaling in breast carcinoma (9), colorectal carcinoma (10), and pancreatic ductal adenocarcinoma (8).
- Sun, W. et al. (2003) Mol Cell Biol 23, 2298-308.
- Kesavan, K. et al. (2004) J Cell Physiol 199, 140-8.
- Cheng, J. et al. (2005) Mol Cell Biol 25, 5955-64.
- Cheng, J. et al. (2005) J Biol Chem 280, 13477-82.
- Takeda, A.N. et al. (2014) EMBO J 33, 1784-801.
- Yamashita, M. et al. (2005) Cell 121, 101-13.
- Zhang, D. et al. (2006) EMBO J 25, 97-107.
- Mazur, P.K. et al. (2014) Nature 510, 283-7.
- Mirza, A.A. et al. (2014) Biochim Biophys Acta 1843, 945-54.
- Jiang, L. et al. (2013) Oncol Lett 6, 1333-37.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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