WB
Vir
Transfected Only
12
Rabbit IgG
#K9N5R3
37616437
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Virus
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro75 of MERS-CoV envelope protein.
Background
Middle East Respiratory Syndrome (MERS) is a contagious viral respiratory infection, whose causative agent was identified as the MERS-related coronavirus (MERS-CoV) (1,2). MERS-CoV is a single-stranded mRNA betacoronavirus. Similar to other infectious coronaviruses (e.g., SARS-CoV-2), the MERS-CoV genome encodes four key structural proteins (spike, envelope, membrane, and nucleocapsid) that make up the virion particle, in addition to at least 10 smaller open reading frames (ORFs) that encode the non-structural (accessory) proteins involved in viral replication (3,4). The MERS envelope protein (E) is the smallest of the structural proteins, and is abundantly expressed in the host cell during viral replication, despite only a fraction of synthesized E protein ultimately being incorporated into new viral particles (5). As in other betacoronaviruses, the MERS E protein likely functions as an ion channel (viroporin), involved in transmembrane molecular transport (6). Within infected host cells, E protein is largely localized to endoplasmic reticulum, Golgi, and related intermediate structures (e.g., ERGIC), consistent with a functionally important role in assembly, budding, and intracellular trafficking of new virion particles (5). This is supported by studies showing that MERS virions were incapable of replication after deletion of the gene encoding the E protein (7).
- Bermingham, A. et al. (2012) Euro Surveill 17, 20290.
- de Groot, R.J. et al. (2013) J Virol 87, 7790-2.
- Javorsky, A. et al. (2021) Commun Biol 4, 724.
- van Boheemen, S. et al. (2012) mBio 3, e00473-12. doi: 10.1128/mBio.00473-12.
- Schoeman, D. and Fielding, B.C. (2019) Virol J 16, 69.
- Barrantes, F.J. (2021) Acta Crystallogr D Struct Biol 77, 391-402.
- Almazán, F. et al. (2013) mBio 4, e00650-13.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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