WB
Vir
Transfected Only
55
Rabbit
#K9N4V7
37616439
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Virus
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro382 of MERS-CoV nucleocapsid protein. Antibodies are purified by peptide affinity chromatography.
Background
Middle East Respiratory Syndrome (MERS) is a contagious viral respiratory infection, whose causative agent was identified as the MERS-related coronavirus (MERS-CoV) (1,2). MERS-CoV is a single-stranded mRNA betacoronavirus. Similar to other infectious coronaviruses (e.g., SARS-CoV-2), the MERS-CoV genome encodes four key structural proteins (spike, envelope, membrane, and nucleocapsid) that make up the virion particle, in addition to at least 10 smaller open reading frames (ORFs) that encode the non-structural (accessory) proteins involved in viral replication (3,4). Nucleocapsid protein is the primary protein involved in packaging the viral genome; however, its functions extend beyond its role in nucleocapsid formation. It may be translationally modified by host enzymes (5,6), and has been reported to influence viral transcription and replication, virion assembly, and the host response to viral infection, through effects on cell cycle, translation, and the innate immune response (7).
- Javorsky, A. et al. (2021) Commun Biol 4, 724.
- de Groot, R.J. et al. (2013) J Virol 87, 7790-2.
- Bermingham, A. et al. (2012) Euro Surveill 17, 20290.
- van Boheemen, S. et al. (2012) mBio 3, e00473-12. doi: 10.1128/mBio.00473-12.
- Surjit, M. et al. (2005) J Virol 79, 11476-86.
- Grunewald, M.E. et al. (2018) Virology 517, 62-68.
- McBride, R. et al. (2014) Viruses 6, 2991-3018.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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