| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| HS1 (D5A9) XP® Rabbit mAb | 3892 | 20 µl | 80 kDa | Rabbit IgG |
| ASC/TMS1 (D2W8U) Rabbit mAb | 67824 | 20 µl | 22 kDa | Rabbit IgG |
| Ki-67 (D3B5) Rabbit mAb | 9129 | 20 µl | Rabbit IgG | |
| MCM2 (D7G11) XP® Rabbit mAb | 3619 | 20 µl | 125 kDa | Rabbit IgG |
| Survivin (71G4B7) Rabbit mAb | 2808 | 20 µl | 16 kDa | Rabbit IgG |
| HP1α/β (C7F11) Rabbit mAb | 2623 | 20 µl | 25 kDa | Rabbit IgG |
| Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP® Rabbit mAb | 2914 | 20 µl | 35, 40, 48 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
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Description
The Microglia Proliferation Module Antibody Sampler Kit provides an economical means of detecting proteins identified as markers of microglial proliferation by western blot and/or immunofluorescence.
Storage
Background
Distinct microglial activation states have been identified using RNA-seq data from a vast array of neurological disease and aging models. These activation states have been categorized into modules corresponding to proliferation, neurodegeneration, interferon-relation, LPS-relation, and many others (1). Previous work identifying markers of specific brain cell types using RNA-seq has shown HS1 and ASC/TMS1 to be useful and specific tools to study microglia (2). HS1 is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (3) and ASC/TMS1 has been found to be a critical component of inflammatory signaling where it associates with and activates caspase-1 in response to pro-inflammatory signals (4).
Ki-67 is a nuclear nonhistone protein (5) universally expressed among proliferating cells and absent in quiescent cells (6). Minichromosome maintenance protein 2 (MCM2) is a nuclear protein that plays a role in DNA replication and cell division (7) and is commonly used as a marker for cell proliferation, including brain tissue (8). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (9). Aurora A, B, and C are a family of highly conserved serine/threonine kinases that regulate chromosomal alignment and segregation during mitosis and meiosis. Their activity requires autophosphorylation of a threonine within their kinase domain at site Thr288 of Aurora A, Thr232 of Aurora B, and Thr198 of Aurora C (10). Heterochromatin protein 1 (HP1) α and β are heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure (11).
- Friedman, B.A. et al. (2018) Cell Rep 22, 832-47.
- Zhang, Y. et al. (2014) J Neurosci 34, 11929-47.
- Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
- Srinivasula, S.M. et al. (2002) J Biol Chem 277, 21119-22.
- Gerdes, J. et al. (1983) Int J Cancer 31, 13-20.
- Weigel, M.T. and Dowsett, M. (2010) Endocr Relat Cancer 17, R245-62.
- Mincheva, A. et al. (1994) Cytogenet Cell Genet 65, 276-7.
- Doorn, K.J. et al. (2014) Neural Plast 2014, 959154.
- Li, F. et al. (1999) Nat Cell Biol 1, 461-6.
- Goldenson, B. and Crispino, J.D. (2015) Oncogene 34, 537-45.
- Maison, C. and Almouzni, G. (2004) Nat Rev Mol Cell Biol 5, 296-304.
Background References
Trademarks and Patents
Limited Uses
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