WB, IP
H
Endogenous
120
Rabbit IgG
#P08235
4306
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human mineralocorticoid receptor protein.
Background
Mineralocorticoid receptor (MR) is a steroid hormone receptor with structural and functional similarities to glucocorticoid receptor (GR). MR binds with high affinity to aldosterone and other mineralocorticoids as well as glucocorticoids (1-3). Upon ligand binding, MR undergoes conformational changes and enters the nucleus to bind to target mineralocorticoid response elements (MREs) (3-5). MR is also able to heterodimerize with GR and bind to hormone response elements on DNA in cells that express both receptors (6-8). Unlike the anti-inflammatory activity of GR, aldosterone and MR can promote cardiovascular inflammation (9,10). Since MR can utilize both aldosterone and glucocorticoids as ligands, selectivity of ligands is facilitated by tissue-specific expression of 11 beta-hydroxysteroid dehydrogenase (11 beta-OHSD). This enzyme converts cortisol to cortisone, a derivative with very little affinity to MR, thus providing a means for tissue-specific MR regulation (11,12). Deficiency of 11 beta-OHSD has shown to cause an excess of MR signaling resulting in hypertension (12,13). Antimineralocorticoid spirolactones have been used as synthetic steroids to induce a transcriptionally inactivated conformational change in MR to treat hypertension and sodium retention (14,15).
- Arriza, J.L. et al. (1987) Science 237, 268-75.
- Giguère, V. et al. (1988) Nature 331, 91-4.
- Beato, M. et al. (1995) Cell 83, 851-7.
- Guiochon-Mantel, A. et al. (1996) J Steroid Biochem Mol Biol 56, 3-9.
- Mangelsdorf, D.J. et al. (1995) Cell 83, 835-9.
- Liu, W. et al. (1995) Proc Natl Acad Sci U S A 92, 12480-4.
- Liu, W. et al. (1996) Mol Endocrinol 10, 1399-406.
- Trapp, T. et al. (1994) Neuron 13, 1457-62.
- Funder, J.W. (1995) J Steroid Biochem Mol Biol 53, 53-5.
- Funder, J.W. Clin Exp Hypertens 19, 885-99.
- Edwards, C.R. et al. (1988) Lancet 2, 986-9.
- Funder, J.W. (1991) Recent Prog Horm Res 47, 191-207; discussion 207-10.
- New, M.I. et al. (1986) Clin Exp Hypertens A 8, 751-72.
- Corvol, P. et al. (1981) Kidney Int 20, 1-6.
- Hellal-Levy, C. et al. (2000) Kidney Int 57, 1250-5.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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