| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 89884T | 1 Kit (5 x 20 microliters) |
|
| Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
|---|---|---|---|---|---|
| MSH2 (D24B5) XP® Rabbit mAb 2017 | 20 µl |
|
H M | 100 | Rabbit IgG |
| MLH1 (D38G9) Rabbit mAb 4256 | 20 µl |
|
H Mk | 84 | Rabbit IgG |
| MSH6 (D60G2) XP® Rabbit mAb 5424 | 20 µl |
|
H Mk | 160 | Rabbit IgG |
| PMS2 (E9U4P) Rabbit mAb 27884 | 20 µl |
|
H | 110 | Rabbit IgG |
| EXO1 (E6P2B) Rabbit mAb 63862 | 20 µl |
|
H M | 120 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody 7074 | 100 µl |
|
Rab | Goat |
Product Information
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro377 of human EXO1 protein, residues surrounding Asn570 of human PMS2 protein, residues near the carboxy terminus of human MSH6 protein, residues near the amino terminus of human MLH1 protein, and residues near the amino terminus of human MSH2 protein.
DNA mismatch repair (MMR), a conserved process for detecting and correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity (1). In prokaryotes, a MutS homodimer recruits a MutL homodimer to sites of DNA mismatches. In eukaryotes, six MutS homologues (MSH1-6) and four MutL homologues (MLH1, PMS2, PMS1, and MLH3) have been identified. Heterodimers composed of two MutL homologues detect distinct DNA mismatch lesions, and heterodimers composed of two MutS homologues perform the repair (2). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases, and DNA ligases (1).
Microsatellite instability (MSI) is a predisposition to genetic mutation resulting from MMR deficiency (dMMR). High MSI (MSI-H) arising from dMMR results in Lynch syndrome, also known as hereditary non-polyposis colorectal cancer (HNPCC). Lynch syndrome is associated with colon cancer, as well as other human cancers (3). MSI and dMMR are strongly associated with tumor responsiveness to immune checkpoint blockade (4,5). MSI status can be determined through PCR amplification of microsatellite markers and/or immunohistochemical detection of MMR proteins MLH1, PMS2, MSH2, and MSH6. The absence of expression of any of these MMR proteins indicates dMMR (3).
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