WB, IHC-P
H M
Endogenous
23
Rabbit IgG
#Q7RTS1
168620
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln181 of human MIST1/bHLHa15 protein.
Background
Class A basic helix-loop-helix protein 15 (MIST1, bHLHa15) is a highly conserved basic helix loop helix family transcription factor that binds E-box motifs and regulates the expression of developmentally regulated genes (1). MIST1 can bind DNA as a homodimer, or may heterodimerize with other bHLH proteins to regulate target gene expression (1). MIST1 is expressed in an array of tissues, including salivary glands, stomach, small intestine, and the pancreas, but is generally restricted to secretory cell subtypes (2). In the pancreas, MIST1 is essential for the maturation, maintenance, and function of acinar cells (3). In gastric chief cells, MIST1 regulates the expression of RAB26 and RAB3D, two GTPases that function to regulate secretory granule formation (4). Loss of MIST1 in gastric chief cells may be a potential marker of gastric epithelial neoplasia (5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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