IHC-P, IF-IC
H
Endogenous
Mouse IgG1 kappa
#O75030
4286
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:3200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a His-tagged fusion protein corresponding to an amino-terminal fragment of human MITF protein (7).
Background
Microphthalmia-associated transcription factor (MITF) is a basic helix-loop-helix leucine zipper transcription factor that is most widely known for its roles in melanocyte, ophthalmic, and osteoclast development (1-3). In humans, MITF can function as a melanoma oncogene (4) and mutations in the corresponding MITF gene are associated with Waardenburg syndrome type 2, an auditory-pigmentary syndrome characterized by developmental defects in cells derived from neural crest (5). At least 12 isoforms of MITF have been identified, which exhibit differential patterns of expression among cell and tissue types (6).
- Hershey, C.L. and Fisher, D.E. (2004) Bone 34, 689-96.
- Nomura, S. et al. (2001) J Bone Miner Metab 19, 183-7.
- Widlund, H.R. and Fisher, D.E. (2003) Oncogene 22, 3035-41.
- Levy, C. et al. (2006) Trends Mol Med 12, 406-14.
- Read, A.P. and Newton, V.E. (1997) J Med Genet 34, 656-65.
- Tachibana, M. (2000) Pigment Cell Res 13, 230-40.
- Hemesath, T.J. et al. (1998) Nature 391, 298-301.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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