Cat. # | Size | Qty. | Price |
---|---|---|---|
74157S | 100 µl |
|
REACTIVITY | H M R Mk |
SENSITIVITY | Endogenous |
MW (kDa) | 135 |
Source/Isotype | Rabbit IgG |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Human, Mouse, Rat, Monkey
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val515 of human MORC2 protein.
Microrchidia family CW-type zinc finger 2 (MORC2) is a protein involved in ATP-dependent chromatin remodeling and DNA repair (1-3). Upon DNA damage, MORC2 dimerizes and is phosphorylated by PAK1. PARP1 can then recruit MORC2 to break sites, where it destabilizes histone-DNA interactions, and recruits DNA repair proteins, such as BRCA1 and Rad51 (4,5). MORC2 is recruited by the HUSH epigenetic silencing complex to sites of heterochromatin (6). MORC2 has also been shown to downregulate important tumor suppressors p21 and ArgBP2 in gastric cancers by recruitment of EZH2 (7-9). Mutations in the MORC2 gene locus have been shown to cause the axonal form of Charcot-Marie-Tooth disease (CMT), a disorder of the motor and sensory neurons of the peripheral nervous system (8-11).
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