Revision 8

#88280

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
FC-FP

Reactivity:
M

Sensitivity:
Endogenous

Source/Isotype:
Rat IgG2b

UniProt ID:
#P08101-2, #P08508

Entrez-Gene Id:
14130, 14131

Product Usage Information

To block the interaction of IgG with mouse Fc receptors, add 1.0 μg of antibody per million cells in 100 µl of buffer. Incubate 10 mins at 4ºC or room temperature as desired. A wash step is not needed prior to adding antibodies of interest. The optimal blocking concentration may be assay dependent and may require titration.
Application Dilution
Flow Cytometry (Fixed/Permeabilized) 1:50 - 1:200

Storage

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Do not freeze.

Specificity/Sensitivity

Mouse Fc Receptor Blocking Solution (CD16/CD32) Rat mAb binds to all isoforms of mouse CD16 and CD32, preventing interaction with the IgG Fc domain.

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography.

Background

The 2.4G2 clone is widely used as a blocking reagent to prevent interactions between mouse Fc receptors and IgG. By binding to CD32 (FcgammaRII) and CD16 (FcgammaRIII), the 2.4G2 clone prevents the Fc receptor proteins from being able to interact with the Fc domain of antibodies subsequently added for analysis. Performing an Fc blocking step thus reduces background and false positive events in flow cytometry assays on live cells.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

M: Mouse

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.