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12222
Mouse IL-3 Neutralizing (D6C1) Rabbit mAb
Primary Antibodies
Monoclonal Antibody

Mouse IL-3 Neutralizing (D6C1) Rabbit mAb #12222

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Mouse IL-3 Neutralizing (D6C1) Rabbit mAb: Image 1

The ability of Mouse IL-3 Neutralizing (D6C1) Rabbit mAb to inhibit mIL-3-induced BaF3 cell proliferation was assessed. Cells were incubated with increasing concentrations of antibody in the presence of mIL-3 #8923 (250 pg/ml). After 72 hr, viable cells were detected by incubation with a tetrazolium salt and the OD450 was determined.

Mouse IL-3 Neutralizing (D6C1) Rabbit mAb: Image 2

The proliferation of BaF3 cells treated with increasing amounts of mIL-3 #8923 was determined. After a 72 hr treatment, cells were incubated with a tetrazolium salt and the OD450 was determined.

To Purchase # 12222S
Product # Size Price
12222S
100 µg $ 355

Supporting Data

REACTIVITY M
SENSITIVITY
MW (kDa)
Source/Isotype Rabbit IgG

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

Neutralizing antibodies can be used to inhibit normal biological function through their binding to biological molecules. These reagents can be used to determine the effects that a particular molecule has in biological systems. Mouse IL-3 Neutralizing (D6C1) Rabbit mAb has been shown to neutralize the proliferation of IL-3-dependent BaF3 cells in vitro with an ND50 in the range of 0.5-3.5 µg/ml.

Product Usage Information

CST recommends incubation of the neutralizing antibody with the intended target for 1 hr at 37ºC before addition to the experiment at an optimal concentration determined by the user.

Reconstitution:

Add sterile 10 mM HEPES pH 7.0 to a final concentration of greater than 50 μg/ml. Solubilize for 20 min at room temperature with occasional gentle vortexing.

Application Dilution
Neutralizing 1:1

Formulation

Lyophilized from a 0.2 µm filtered solution in 10 mM HEPES with trehalose.

Storage

Store lyophilized material at -20ºC. After reconstitution, recommended storage at 4ºC for 1 month or -20ºC for 6 months. Avoid repeated freeze/thawing.

Specificity / Sensitivity

Mouse IL-3 Neutralizing (D6C1) Rabbit mAb binds to mouse IL-3 and neutralizes its effects in a BaF3 cell proliferation assay. This antibody does not cross-react with human IL-3, human IL-5, human GM-CSF, or mouse GM-CSF.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant mouse IL-3 protein.

Background

IL-3 is produced by T cells, mast cells, and eosinophils (1). Target cells include hematopoietic progenitors, neutrophils, macrophages, mast cells, eosinophils, lymphoid, and erythroid cells (1). IL-3 supports growth and differentiation and is used as a media additive to support the culture of many cell types (1). The IL-3 receptor is a heterodimer of the IL-3-specific α-chain and the common β-chain, βc, which is also used by GM-CSF and IL-5. (1). Binding of IL-3 can also involve substitution of βc by a βIL-3-chain that appears to be specific for IL-3 (1,2). Binding of IL-3 to its cognate receptor(s) induces activation of Jak2 and the PI3K/Akt pathway, and phosphorylation of Stat1/3/5/6 (1). IL-3 may play an important role in the development of airway inflammation associated with asthma (3-5).

  1. Reddy, E.P. et al. (2000) Oncogene 19, 2532-47.
  2. Hara, T. and Miyajima, A. (1992) EMBO J 11, 1875-84.
  3. Asquith, K.L. et al. (2008) J Immunol 180, 1199-206.
  4. Schroeder, J.T. et al. (2009) J Immunol 182, 2432-8.
  5. Munitz, A. et al. (2006) J Immunol 177, 77-83.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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