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37495
Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit #37495

Citations (1)
Immunohistochemical analysis of paraffin-embedded 4T1 metastatic tumor in mouse lung using FoxP3 (D6O8R) Rabbit mAb performed on the Leica® BOND Rx.
Western blot analysis of extracts from various cell lines and tissues using CD4 (D7D2Z) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from various mouse and human cells using Granzyme B (E5V2L) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, Granzyme B (E5V2L) Rabbit mAb only detects Granzyme B in mouse primary CD8+ T cells and mouse T cell lines. CD8+ T cells were purified from mouse spleens or human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Mouse Interleukin-2 (mIL-2) #5201 (20 ng/ml) or Human Interleukin-2 (hIL-2) #8907 (20 ng/ml). KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
Western blot analysis of extracts from various cell lines using F4/80 (D2S9R) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from mouse CD4+ T cells, EL4, and C2C12 cells using CD3ε (E4T1B) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). CD4+ T cells were purified from mouse spleens and stimulated for 9 days using beads coated with mouse CD3 and CD28 antibodies in the presence of Mouse Interleukin-2 (mIL-2) #5201 (20 ng/ml).
Western blot analysis of extracts from various cell lines using CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, CD19 protein is not detected in either U-937 cells or Raw 264.7 cells.
Western blot analysis of extracts from Raw 264.7, C2C12, and 3T3 cells using CD11c (D1V9Y) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from mouse CD8+ T cells, mouse spleen, EL4 cells, and Raw264.7 cells using CD8α (D4W2Z) XP® Rabbit mAb (upper), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunohistochemical analysis of paraffin-embedded mouse thymus using FoxP3 (D6O8R) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded 4T1 metastatic tumor in mouse lung using CD4 (D7D2Z) Rabbit mAb performed on the Leica® BOND Rx.
Immunohistochemical analysis of paraffin-embedded CT26.WT syngeneic tumor using Granzyme B (E5V2L) Rabbit mAb performed on the Leica® BOND Rx.
Immunohistochemical analysis of paraffin-embedded mouse liver using F4/80 (D2S9R) XP® Rabbit mAb.
Immunoprecipitation of CD3ε protein from EL4 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CD3ε (E4T1B) XP® Rabbit mAb. Western blot analysis was performed using CD3ε (E4T1B) XP® Rabbit mAb.
Western blot analysis of extracts from Daudi cells, untreated (-) or treated with PNGase F (+), using CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunohistochemical analysis of paraffin-embedded 4T1 mammary tumor using CD11c (D1V9Y) Rabbit mAb performed on the Leica® Bond Rx.
Immunohistochemical analysis of paraffin-embedded mouse lung using CD8α (D4W2Z) XP® Rabbit mAb performed on the Leica® BOND Rx.
Immunohistochemical analysis of paraffin-embedded mouse spleen using FoxP3 (D6O8R) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse colon using CD4 (D7D2Z) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mammary tumor using Granzyme B (E5V2L) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse spleen using F4/80 (D2S9R) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse lung using CD3ε (E4T1B) XP® Rabbit mAb performed on the Leica® BOND Rx.
Immunohistochemical analysis of paraffin-embedded human endometrioid carcinoma using CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb performed on the Leica® Bond Rx.
Immunohistochemical analysis of paraffin-embedded mouse kidney using CD11c (D1V9Y) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD8α (D4W2Z) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse small intestine using FoxP3 (D6O8R) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD4 (D7D2Z) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse small intestine using Granzyme B (E5V2L) Rabbit mAb (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemical analysis of paraffin-embedded mouse small intestine using F4/80 (D2S9R) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mammary tumor using CD3ε (E4T1B) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse liver using CD11c (D1V9Y) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD8α (D4W2Z) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse lung using FoxP3 (D6O8R) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD4 (D7D2Z) Rabbit mAb.
Flow cytometric analysis of C2C12 cells (blue, negative) and CTLL-2 cells (green, positive) using Granzyme B (E5V2L) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Immunohistochemical analysis of paraffin-embedded Raw 264.7 cell pellet (left, positive) or Neuro-2A cell pellet (right, negative) using F4/80 (D2S9R) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using CD3ε (E4T1B) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse lung using CD11c (D1V9Y) Rabbit mAb.
Multiplex immunohistochemical analysis of paraffin-embedded mouse LL2 syngeneic tumor tissue using CD8α (D4W2Z) XP® rabbit mAb (magenta), F4/80 (D2S9R) XP® rabbit mAb #70076 (orange), PD-1 (D7D5W) XP® rabbit mAb #84651 (green), PD-L1 (D5V3B) rabbit mAb #64988 (yellow), CD3ε (D4V8L) rabbit mAb #99940 (red) and pan keratin (C11) mouse mAb #4279 (cyan).
Immunohistochemical analysis of paraffin-embedded normal rhesus monkey spleen using FoxP3 (D6O8R) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded rat lung using CD4 (D7D2Z) Rabbit mAb.
Multiplex immunohistochemical analysis of paraffin-embedded mouse LL2 syngeneic tumor tissue using F4/80 (D2S9R) XP® rabbit mAb (orange), PD-1 (D7D5W) XP® rabbit mAb #84651 (green), PD-L1 (D5V3B) rabbit mAb #64988 (yellow), CD3ε (D4V8L) rabbit mAb #99940 (red), CD8α (D4W2Z) XP® rabbit mAb #98941 (magenta) and pan keratin (C11) mouse mAb #4279 (cyan).
Immunohistochemical analysis of paraffin-embedded MC38 syngeneic tumor using CD3ε (E4T1B) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lymphoma using CD19 (Intraceullular Domain) (D4V4B) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded Raw 264.7 cell pellet (left, positive) or C2C12 cell pellet (right, negative) using CD11c (D1V9Y) Rabbit mAb.
Immunofluorescent analysis of mouse spleen using FoxP3 (D6O8R) Rabbit mAb (red) and a CD4 antibody (green). Blue = DRAQ5® #4084 (fluorescent DNA dye).Note: FoxP3 (D6O8R) Rabbit mAb has been validated on freshly-prepared frozen tissue sections that were fixed for 15 minutes in 4% formaldehyde. Over-fixation diminishes the performance of this antibody.
Immunohistochemical analysis of paraffin-embedded Syrian hamster small intestine using CD4 (D7D2Z) Rabbit mAb.
Immunoprecipitation of F4/80 from BaF3 cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is F4/80 (D2S9R) XP® Rabbit mAb, #70076. Western blot was performed using F4/80 (D2S9R) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD3ε (E4T1B) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemical analysis of paraffin-embedded human tonsil using CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD11c (D1V9Y) Rabbit mAb.
Flow cytometric analysis of mouse splenocytes using FoxP3 (D6O8R) Rabbit mAb (right) and co-stained with CD4 (RM4-5) Rat mAb (APC Conjugate) #82116, compared to a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) was used as a secondary antibody.
Confocal immunofluorescent analysis of mouse spleen (left, positive) or pancreas (right, negative) using CD3ε (E4T1B) XP® Rabbit mAb (red). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using HS1 (D5A9) XP® Rabbit mAb (Rodent Specific) (Alexa Fluor® 488 Conjugate) #68206 (green). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb.
Confocal immunofluorescent analysis of mouse spleen (left) and mouse kidney (right) using CD11c (D1V9Y) Rabbit mAb. Nuclei were labeled with ProLong® Gold Antifade reagent with DAPI #8961 (blue).
Flow cytometric analysis of U-937 (negative, blue) and Raji (positive, green) cells using CD19 (D4V4B) Rabbit mAb (solid line) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.
Confocal immunofluorescent analysis of Raw 264.7 cells (left, positive) and 3T3 cells (right, negative) using CD11c (D1V9Y) Rabbit mAb (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.
Flow cytometric analysis of Raw264.7 (negative, blue) and A20 (positive, green) cells using CD19 (D4V4B) Rabbit mAb (solid line) or a concentration-matched Rabbit DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.
To Purchase # 37495
Cat. # Size Qty. Price
37495T
1 Kit  (8 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
CD4 (D7D2Z) Rabbit mAb 25229 20 µl
  • WB
  • IHC
M R Hm 55 Rabbit IgG
CD8α (D4W2Z) XP® Rabbit mAb 98941 20 µl
  • WB
  • IHC
M 35-42 Rabbit IgG
FoxP3 (D6O8R) Rabbit mAb 12653 20 µl
  • IHC
  • IF
  • F
M Mk Rabbit IgG
F4/80 (D2S9R) XP® Rabbit mAb 70076 20 µl
  • WB
  • IP
  • IHC
M 65-250 Rabbit IgG
CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb 90176 20 µl
  • WB
  • IP
  • IHC
  • F
H M Mk 95 Rabbit IgG
CD11c (D1V9Y) Rabbit mAb 97585 20 µl
  • WB
  • IHC
  • IF
M 145 Rabbit IgG
Granzyme B (E5V2L) Rabbit mAb 44153 20 µl
  • WB
  • IHC
  • F
M 30 Rabbit IgG
CD3ε (E4T1B) XP® Rabbit mAb 78588 20 µl
  • WB
  • IP
  • IHC
  • IF
M 23 Rabbit IgG

Product Description

The Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit provides an economical means of detecting the accumulation of immune cell types in formalin-fixed, paraffin-embedded tissue samples.

Specificity / Sensitivity

Each antibody included in the Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit recognizes endogenous levels of its target mouse protein. Non-specific staining has been observed in mouse kidney and liver with CD4 (D7D2Z) Rabbit mAb, and in mouse kidney with Granzyme B (E5V2L) Rabbit mAb (Mouse Specific).

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Leu50 of mouse CD3ε protein, Asp42 of mouse CD8α protein, Ala232 of mouse CD4 protein, Pro44 of mouse FoxP3 protein, Leu427 of human CD19 protein, Ala1153 of mouse CD11c protein, or with a synthetic peptide corresponding to residues surrounding Ala197 of mouse Granzyme B protein or mouse F4/80 protein.

Background

Cluster of Differentiation 3 (CD3) is a multiunit protein complex expressed on the surface of T-cells that directly associates with the T-cell receptor (TCR). CD3 is composed of four polypeptides: ζ, γ, ε and δ. Engagement of TCR complex with antigens presented in Major Histocompatibility Complexes (MHC) induces tyrosine phosphorylation in the immunoreceptor tyrosine-based activation motif (ITAM) of CD3 proteins. CD3 phosphorylation is required for downstream signaling through ZAP-70 and p85 subunit of PI-3 kinase, leading to T cell activation, proliferation, and effector functions (1). Cluster of Differentiation 8 (CD8) is a transmembrane glycoprotein expressed primarily on cytotoxic T cells, but has also been described on a subset of dendritic cells in mice (2,3). On T cells, CD8 is a co-receptor for the TCR, and these two distinct structures are required to recognize antigen bound to MHC Class I (2). Cluster of Differentiation 4 (CD4) is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the TCR, and these two distinct structures recognize antigen bound to MHC Class II. CD8 and CD4 co-receptors ensure specificity of the TCR–antigen interaction, prolong the contact between the T cell and the antigen presenting cell, and recruit the tyrosine kinase Lck, which is essential for T cell activation (2). Granzyme B is a serine protease expressed by CD8+ cytotoxic T lymphocytes and natural killer (NK) cells and is a key component of the immune response to pathogens and transformed cancer cells (4). Forkhead box P3 (FoxP3) is crucial for the development of T cells with immunosuppressive regulatory properties and is a well-established marker for T regulatory cells (Tregs) (5). CD19 is a co-receptor expressed on B cells that amplifies the signaling cascade initiated by the B cell receptor (BCR) to induce activation. It is a biomarker of B lymphocyte development, lymphoma diagnosis, and can be utilized as a target for leukemia immunotherapies (6,7). F4/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (8). CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein highly expressed by dendritic cells, and has also been observed on activated NK cells, subsets of B and T cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (9,10).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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