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37495
Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit #37495

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Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 1

Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD3ε (D4V8L) Rabbit mAb performed on the Leica® BOND™ Rx.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 2

Immunohistochemical analysis of paraffin-embedded 4T1 metastatic tumor in mouse lung using CD4 (D7D2Z) Rabbit mAb performed on the Leica® BOND™ Rx.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 3

Immunohistochemical analysis of paraffin-embedded mouse lung using CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific) performed on the Leica® BOND™ Rx.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 4

Flow cytometric analysis of mouse splenocytes using FoxP3 (D6O8R) Rabbit mAb (right) and co-stained with CD4 (RM4-5) Rat mAb (APC Conjugate) #82116, compared to a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) was used as a secondary antibody.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 5

Immunofluorescent analysis of mouse spleen using FoxP3 (D6O8R) Rabbit mAb (red) and a CD4 antibody (green). Blue = DRAQ5® #4084 (fluorescent DNA dye).

Note: FoxP3 (D6O8R) Rabbit mAb has been validated on freshly-prepared frozen tissue sections that were fixed for 15 minutes in 4% formaldehyde. Over-fixation diminishes the performance of this antibody.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 6

Immunohistochemical analysis of paraffin-embedded 4T1 metastatic tumor in mouse lung using FoxP3 (D6O8R) Rabbit mAb performed on the Leica® BOND™ Rx.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 7

Immunohistochemical analysis of paraffin-embedded mouse liver using F4/80 (D2S9R) XP® Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 8

Immunohistochemical analysis of paraffin-embedded human endometrioid carcinoma using CD19 (D4V4B) XP® Rabbit mAb performed on the Leica® Bond™ Rx.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 9

Immunohistochemical analysis of paraffin-embedded 4T1 mammary tumor using CD11c (D1V9Y) Rabbit mAb performed on the Leica® Bond™ Rx.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 10

Confocal immunofluorescent analysis of mouse spleen (left) and mouse kidney (right) using CD11c (D1V9Y) Rabbit mAb. Nuclei were labeled with ProLong® Gold Antifade reagent with DAPI #8961 (blue).

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 11

Immunohistochemical analysis of paraffin-embedded human colon adenocarinoma using Granzyme B (D6E9W) Rabbit mAb performed on the Leica® BOND™ Rx.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 12

Immunohistochemical analysis of paraffin-embedded mouse spleen using CD3ε (D4V8L) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 13

Immunohistochemical analysis of paraffin-embedded mouse colon using CD4 (D7D2Z) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 14

Immunohistochemical analysis of paraffin-embedded mouse spleen using CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific).

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 15

Immunohistochemical analysis of paraffin-embedded mouse thymus using FoxP3 (D6O8R) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 16

Immunohistochemical analysis of paraffin-embedded mouse spleen using F4/80 (D2S9R) XP® Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 17

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD19 (D4V4B) XP® Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 18

Confocal immunofluorescent analysis of Raw 264.7 cells (left, positive) and 3T3 cells (right, negative) using CD11c (D1V9Y) Rabbit mAb (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 19

Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD3ε (D4V8L) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 20

Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD4 (D7D2Z) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 21

Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific).

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 22

Immunohistochemical analysis of paraffin-embedded mouse spleen using FoxP3 (D6O8R) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 23

Immunohistochemical analysis of paraffin-embedded mouse small intestine using F4/80 (D2S9R) XP® Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 24

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CD19 (D4V4B) XP® Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 25

Immunohistochemical analysis of paraffin-embedded human papillary carcinoma of the breast using Granzyme B (D6E9W) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 26

Immunohistochemical analysis of paraffin-embedded mouse lung using CD3ε (D4V8L) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 27

Immunohistochemical analysis of paraffin-embedded mouse spleen using CD4 (D7D2Z) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 28

Immunohistochemical analysis of paraffin-embedded mouse small intestine using FoxP3 (D6O8R) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 29

Immunohistochemical analysis of paraffin-embedded Raw 264.7 cell pellet (left, positive) or Neuro-2A cell pellet (right, negative) using F4/80 (D2S9R) XP® Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 30

Immunohistochemical analysis of paraffin-embedded human lymphoma using CD19 (D4V4B) XP® Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 31

Immunohistochemical analysis of paraffin-embedded mouse kidney using CD11c (D1V9Y) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 32

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Granzyme B (D6E9W) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 33

Multiplex immunohistochemical analysis of paraffin-embedded mouse LL2 syngeneic tumor tissue using CD3ε (D4V8L) Rabbit mAb (red), F4/80 (D2S9R) XP® Rabbit mAb #70076 (orange), PD-1 (D7D5W) XP® Rabbit mAb #84651 (green), PD-L1 (D5V3B) Rabbit mAb #64988 (yellow), CD8α (D4W2Z) XP® Rabbit mAb #98941 (magenta) and Pan-Keratin (C11) Mouse mAb (Biotinylated) #4279 (cyan). Note: Biotinylated Pan-Keratin (C11) Mouse mAb was used in order to avoid mouse on mouse background staining.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 34

Immunohistochemical analysis of paraffin-embedded rat lung using CD4 (D7D2Z) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 35

Western blot analysis of extracts from mouse CD8+ T cells, mouse spleen, EL4 cells, and Raw264.7 cells using CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific) (upper), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 36

Immunohistochemical analysis of paraffin-embedded mouse lung using FoxP3 (D6O8R) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 37

Immunohistochemical analysis of paraffin-embedded mouse spleen using CD19 (D4V4B) XP® Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 38

Immunohistochemical analysis of paraffin-embedded mouse liver using CD11c (D1V9Y) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 39

Immunohistochemical analysis of paraffin-embedded KARPAS-299 cell pellet (left, positive) or Jurkat cell pellet (right, negative) using Granzyme B (D6E9W) Rabbit mAb. KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 40

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using Granzyme B (D6E9W) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 41

Western blot analysis of extracts from mouse spleen, EL4 cells, and C2C12 cells using CD3ε (D4V8L) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 42

Western blot analysis of extracts from various cell lines and tissues using CD4 (D7D2Z) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 43

Western blot analysis of extracts from various cell lines using F4/80 (D2S9R) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 44

Immunohistochemical analysis of paraffin-embedded human tonsil using CD19 (D4V4B) XP® Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 45

Immunohistochemical analysis of paraffin-embedded mouse lung using CD11c (D1V9Y) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 46

Western blot analysis of extracts from various human cells using Granzyme B (D6E9W) Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). CD8+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of human interleukin-2 (hIL-2) #8907 (20 ng/ml). KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 47

Western blot analysis of extracts from various cell lines using CD19 (D4V4B) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, CD19 protein is not detected in either U-937 cells or Raw 264.7 cells.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 48

Immunohistochemical analysis of paraffin-embedded Raw 264.7 cell pellet (left, positive) or C2C12 cell pellet (right, negative) using CD11c (D1V9Y) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 49

Western blot analysis of extracts from CTLL-2, mouse CD8+ T, and C2C12 cells using Granzyme B (D6E9W) Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). CD8+ T cells were purified from mouse spleens and stimulated for 9 days using beads coated with mouse CD3 and CD28 antibodies in the presence of Mouse Interleukin-2 (mIL-2) #5201 (20 ng/ml).

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 50

Western blot analysis of extracts from Daudi cells, untreated (-) or treated with PNGase F (+), using CD19 (D4V4B) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 51

Immunohistochemical analysis of paraffin-embedded mouse spleen using CD11c (D1V9Y) Rabbit mAb.

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 52

Western blot analysis of extracts from Raw 264.7, C2C12, and 3T3 cells using CD11c (D1V9Y) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 37495T
Product # Size Price
37495T
1 Kit  (8 x 20 µl) $ 640

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
CD3ε (D4V8L) Rabbit mAb 99940 20 µl
  • WB
  • IHC
M 22 Rabbit IgG
CD4 (D7D2Z) Rabbit mAb 25229 20 µl
  • WB
  • IHC
M R 55 Rabbit IgG
CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific) 98941 20 µl
  • WB
  • IHC
M 35-42 Rabbit IgG
FoxP3 (D6O8R) Rabbit mAb 12653 20 µl
  • IHC
  • IF
  • F
M Rabbit IgG
F4/80 (D2S9R) XP® Rabbit mAb 70076 20 µl
  • WB
  • IP
  • IHC
M 65-250 Rabbit IgG
CD19 (D4V4B) XP® Rabbit mAb 90176 20 µl
  • WB
  • IP
  • IHC
H M 95 Rabbit IgG
CD11c (D1V9Y) Rabbit mAb 97585 20 µl
  • WB
  • IHC
  • IF
M 145 Rabbit IgG
Granzyme B (D6E9W) Rabbit mAb 46890 20 µl
  • WB
  • IHC
H M 30 Rabbit IgG

Product Description

The Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit provides an economical means of detecting the accumulation of immune cell types in formalin-fixed, paraffin-embedded tissue samples.

Specificity / Sensitivity

Each antibody included in the Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit recognizes endogenous levels of its target mouse protein. Non-specific staining has been observed in mouse pancreas with CD3ε (D4V8L) Rabbit mAb, in mouse kidney and liver with CD4 (D7D2Z) Rabbit mAb, and in the sweat glands with Granzyme B (D6E9W) Rabbit mAb.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Val31 of mouse CD3ε protein, Asp42 of mouse CD8α protein, Ala232 of mouse CD4 protein, Pro44 of mouse FoxP3 protein, Leu427 of human CD19 protein, Ala1153 of mouse CD11c protein, or with recombinant proteins specific to human Granzyme B protein or mouse F4/80 protein.

Background

Cluster of Differentiation 3 (CD3) is a multiunit protein complex expressed on the surface of T-cells that directly associates with the T-cell receptor (TCR). CD3 is composed of four polypeptides: ζ, γ, ε and δ. Engagement of TCR complex with antigens presented in Major Histocompatibility Complexes (MHC) induces tyrosine phosphorylation in the immunoreceptor tyrosine-based activation motif (ITAM) of CD3 proteins. CD3 phosphorylation is required for downstream signaling through ZAP-70 and p85 subunit of PI-3 kinase, leading to T cell activation, proliferation, and effector functions (1). Cluster of Differentiation 8 (CD8) is a transmembrane glycoprotein expressed primarily on cytotoxic T cells, but has also been described on a subset of dendritic cells in mice (2,3). On T cells, CD8 is a co-receptor for the TCR, and these two distinct structures are required to recognize antigen bound to MHC Class I (2). Cluster of Differentiation 4 (CD4) is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the TCR, and these two distinct structures recognize antigen bound to MHC Class II. CD8 and CD4 co-receptors ensure specificity of the TCR–antigen interaction, prolong the contact between the T cell and the antigen presenting cell, and recruit the tyrosine kinase Lck, which is essential for T cell activation (2). Granzyme B is a serine protease expressed by CD8+ cytotoxic T lymphocytes and natural killer (NK) cells and is a key component of the immune response to pathogens and transformed cancer cells (4). Forkhead box P3 (FoxP3) is crucial for the development of T cells with immunosuppressive regulatory properties and is a well-established marker for T regulatory cells (Tregs) (5). CD19 is a co-receptor expressed on B cells that amplifies the signaling cascade initiated by the B cell receptor (BCR) to induce activation. It is a biomarker of B lymphocyte development, lymphoma diagnosis, and can be utilized as a target for leukemia immunotherapies (6,7). F4/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (8). CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein highly expressed by dendritic cells, and has also been observed on activated NK cells, subsets of B and T cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (9,10).

  1. Kuhns, M.S. et al. (2006) Immunity 24, 133-139.
  2. Zamoyska, R. (1994) Immunity 1, 243-46.
  3. Shortman, K. and Heath, W.R. (2010) Immunol Rev 234, 18-31.
  4. Trapani, J.A. (2001) Genome Biol. 2, REVIEWS 3014.
  5. Ochs, H.D. et al. (2007) Immunol Res 38, 112-21.
  6. Tedder, T.F. et al. (1997) Immunity 6, 107-18.
  7. Scheuermann, R.H. and Racila, E. (1995) Leuk Lymphoma 18, 385-97.
  8. McKnight, A.J. et al. (1996) J Biol Chem 271, 486-9.
  9. Kohrgruber, N. et al. (1999) J Immunol 163, 3250-9.
  10. Qualai, J. et al. (2016) PLoS One 11, e0154253.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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