| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| NLRP3 (D4D8T) Rabbit mAb | 15101 | 20 µl | 110 kDa | Rabbit IgG |
| AIM2 Antibody | 63660 | 20 µl | 43 kDa | Rabbit |
| ASC/TMS1 (D2W8U) Rabbit mAb | 67824 | 20 µl | 22 kDa | Rabbit IgG |
| Cleaved-IL-1β (Asp117) (E7V2A) Rabbit mAb | 63124 | 20 µl | 17 kDa | Rabbit IgG |
| IL-1β (D6D6T) Rabbit mAb | 31202 | 20 µl | 17, 31 kDa | Rabbit IgG |
| Cleaved Caspase-1 (Asp296) (E2G2I) Rabbit mAb | 89332 | 20 µl | 22 kDa | Rabbit IgG |
| Caspase-1 (E2Z1C) Rabbit mAb | 24232 | 20 µl | 48, 10 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Mouse Reactive Inflammasome Antibody Sampler Kit provides an economical means of detecting multiple inflammasome components. The kit includes enough antibodies to perform at least two western blot experiments with each primary antibody.
Storage
Background
The innate immune system works as the first line of defense in protection from pathogenic microbes and host-derived signals of cellular distress. One way in which these “danger” signals trigger inflammation is through activation of inflammasomes, which are multiprotein complexes that assemble in the cytosol after exposure to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) and result in the activation of caspase-1 and subsequent cleavage of proinflammatory cytokines IL-1β and IL-18 (Reviewed in 1-6). Inflammasome complexes typically consist of a cytosolic pattern recognition receptor (PRR; a nucleotide-binding domain and leucine-rich-repeat [NLR] or AIM2-like receptor [ALR] family member), an adaptor protein (ASC/TMS1), and pro-caspase-1. A number of distinct inflammasome complexes have been identified, each with a unique PRR and activation triggers. The best characterized is the NLRP3 complex, which contains NLRP3, ASC/TMS1, and pro-caspase-1. The NLRP3 inflammasome is activated in a two-step process. First, NF-κB signaling is induced through PAMP- or DAMP-mediated activation of TLR4 or TNFR, resulting in increased expression of NLRP3, pro-IL-1β, and pro-IL-18 (priming step, signal 1). Next, indirect activation of NLRP3 occurs by a multitude of signals (whole pathogens, PAMPs/DAMPs, potassium efflux, lysosomal-damaging environmental factors [uric acid, silica, alum] and endogenous factors [amyloid-β, cholesterol crystals], and mitochondrial damage), leading to complex assembly and activation of caspase-1 (signal 2). The complex inflammasome structure is built via domain interactions among the protein components. Other inflammasomes are activated by more direct means: double-stranded DNA activates the AIM2 complex, anthrax toxin activates NLRP1, and bacterial flagellin activates NLRC4. Activated caspase-1 induces secretion of proinflammatory cytokines IL-1β and -18, but also regulates metabolic enzyme expression, phagosome maturation, vasodilation, and pyroptosis, an inflammatory programmed cell death. Inflammasome signaling contributes to the onset of a number of diseases, including atherosclerosis, type II diabetes, Alzheimer’s disease, and autoimmune disorders.
- Broz, P. and Dixit, V.M. (2016) Nat Rev Immunol 16, 407-20.
- Guo, H. et al. (2015) Nat Med 21, 677-87.
- Jo, E.K. et al. (2016) Cell Mol Immunol 13, 148-59.
- Rathinam, V.A. and Fitzgerald, K.A. (2016) Cell 165, 792-800.
- Shao, B.Z. et al. (2015) Front Pharmacol 6, 262.
- Schroder, K. and Tschopp, J. (2010) Cell 140, 821-32.
Background References
Trademarks and Patents
Limited Uses
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