| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| F4/80 (D2S9R) XP® Rabbit mAb | 70076 | 20 µl | 65-250 kDa | Rabbit IgG |
| CD68 (E3O7V) Rabbit mAb | 97778 | 20 µl | 70-80, 130-140, 200 kDa | Rabbit IgG |
| CD86 (E5W6H) Rabbit mAb | 19589 | 20 µl | 60-85 kDa | Rabbit IgG |
| CD11c (D1V9Y) Rabbit mAb | 97585 | 20 µl | 145 kDa | Rabbit IgG |
| CD206/MRC1 (E6T5J) XP® Rabbit mAb | 24595 | 20 µl | 190-250 kDa | Rabbit IgG |
| Arginase-1 (D4E3M™) XP® Rabbit mAb | 93668 | 20 µl | 40 kDa | Rabbit IgG |
| Iba1/AIF-1 (E4O4W) XP® Rabbit mAb | 17198 | 20 µl | 17 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit provides an economical means of characterizing the extent of M1 and M2 macrophages in formalin-fixed, paraffin-embedded tissue samples.
Storage
Background
Macrophages are myeloid cells of the innate immune system that are found in all human tissues in the body and exhibit anatomical and functional diversity. These heterogenous cells are derived from monocyte precursors in the blood that infiltrate into the tissues and differentiate in the presence of cytokines and growth factors. A spectrum of different macrophage phenotypes, or polarizations, have been described based on their secretory profiles, gene expression, and functions. Macrophages have great plasticity and can switch from one phenotype to another under different conditions. At the opposite extremes of this spectrum are so called M1, or classically activated phenotype, and M2 or alternatively activated phenotype. M1 macrophages are generally inflammatory and anti-tumor, while M2 macrophages, commonly referred to as tumor-associated macrophages (TAMs), are generally anti-inflammatory and pro-tumor. Relative contents of M1 and M2 macrophages in the tumor microenvironment may have prognostic values. Modulating macrophage polarization is actively pursued as a therapeutic intervention for many different diseases (1-6).
In mice, F4/80, CD68, and Iba1/AIF-1 are considered general markers for macrophages. CD86, CD11c, and others have been used as markers for M1 macrophages, while CD206, Arginase-1, and others have been used as markers for M2 macrophages (7-10).
- Wynn, T.A. et al. (2013) Nature 496, 445-55.
- Biswas, S.K. and Mantovani, A. (2010) Nat Immunol 11, 889-96.
- Mills, C.D. (2012) Crit Rev Immunol 32, 463-88.
- Wang, N. et al. (2014) Front Immunol 5, 614.
- Orecchioni, M. et al. (2019) Front Immunol 10, 1084.
- Yunna, C. et al. (2020) Eur J Pharmacol 877, 173090.
- Kim, H.M. et al. (2017) PLoS One 12, e0179204.
- Gensel, J.C. et al. (2017) Sci Rep 7, 40144.
- Li, X. et al. (2018) Front Pharmacol 9, 72.
- Yang, J. et al. (2018) Cereb Cortex 28, 2622-2635.
Background References
Trademarks and Patents
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
