| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| TREM2 (E7P8J) Rabbit mAb (Carboxy-terminal Antigen) | 76765 | 20 µl | 11, 28 kDa | Rabbit IgG |
| TREM2 (E6T1P) Rabbit mAb (Amino-terminal Antigen) | 61788 | 20 µl | 28 kDa | Rabbit IgG |
| Syk (D3Z1E) XP® Rabbit mAb | 13198 | 20 µl | 72 kDa | Rabbit IgG |
| Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb | 2717 | 20 µl | 70, 72 kDa | Rabbit IgG |
| DAP12 (D7G1X) Rabbit mAb | 12492 | 20 µl | 10, 12 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
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Description
The Mouse TREM2 Activity Antibody Sampler Kit provides an economical means of evaluating key members of the mouse TREM2 signaling pathway using phospho-specific and control antibodies. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Storage
Background
Alzheimer's Disease (AD) is one of the most common neurodegenerative diseases worldwide. Clinically, it is characterized by the presence of extracellular amyloid plaques and intracellular neurofibrillary tangles, resulting in neuronal dysfunction and cell death. Triggering receptor expressed on myeloid cells 2 (TREM2), a protein localized at the membrane of innate immune cells, including microglia in the brain, has been genetically linked to AD, with specific variants increasing disease risk by as much as threefold (1,2). The TREM2 receptor is a single-pass type I membrane glycoprotein that consists of an extracellular immunoglobulin-like domain, a transmembrane domain, and a cytoplasmic tail. Upon activation, TREM2 interacts with the tyrosine kinase-binding protein DNAX-activating protein 12 (DAP12, TYROBP) to form a receptor-signaling complex. The DAP12 protein structure consists of a short extracellular domain, a transmembrane domain, and a cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM) (2-9). ITAMs function as a binding site for tyrosine kinases, including spleen tyrosine kinase (Syk). Syk is comprised of two tandem amino-terminal Src homology (SH) 2 domains separated by an SH2-kinase linker, and a C-terminal tyrosine kinase domain, separated from the SH2 domains by an inter-domain linker. When Syk binds to an ITAM, it changes conformation, allowing for residues within the inter-domain linker region, including Tyr352, to become phosphorylated. Residues within the activation loop subsequently become phosphorylated, leading to full Syk activation. Tyr525 and Tyr526 are located in the activation loop of the Syk kinase domain and phosphorylation at these residues (equivalent to Tyr519/520 of mouse Syk) is essential for Syk function (10-12). This activation can lead to the mediation of a variety of cellular responses, including proliferation, differentiation, inflammation, and phagocytosis. Evidence suggests that TREM2 and DAP12 may act in a Syk-dependent manner to drive microglial cellular responses in AD (2,4-8,13).
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Background References
Trademarks and Patents
Limited Uses
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