WB, IP
H
Endogenous
160-220
Rabbit IgG
#O15438
8714
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein surrounding Gly870 of human MRP3 protein.
Background
MRP3/ABCC3 belongs to the super family of ATP-binding cassette (ABC) transporters. It is a member of the MRP subfamily that is expressed in various organs including liver, gallbladder, small intestine, colon, kidney, and adrenal gland (1-3). MRP3 is involved in multi-drug resistance (1). It facilitates the efflux of organic anions including monoanionic bile acid and anti-cancer reagents such as etoposide and paclitaxel from liver and small intestine into blood (4-7). Expression of MRP3 is increased in the cholestatic human and rat liver, suggesting its role in cholehepatic and enterohepatic bile circulation and in protecting liver from toxic bile salts (2,8). MRP3 expression is also upregulated in people with Dubin-Johnson Syndrome (DJS) who lack functional MRP2 in the liver, which implicates the compensatory role of MRP3 in the absence of functional MRP2 (4).
Elevated expression of MRP3 has been detected in various cancer types such as hepatocellular carcinomas, primary ovarian cancer, and adult acute lymphoblastic leukemia (ALL) (9-11). Overexpression of MRP3 was reported to be a prognostic factor in ALL and adult acute myeloid leukemia (AML) (11,12).
- Uchiumi, T. et al. (1998) Biochem Biophys Res Commun 252, 103-10.
- Scheffer, G.L. et al. (2002) Lab Invest 82, 193-201.
- Kiuchi, Y. et al. (1998) FEBS Lett 433, 149-52.
- König, J. et al. (1999) Hepatology 29, 1156-63.
- Zelcer, N. et al. (2001) J Biol Chem 276, 46400-7.
- Belinsky, M.G. et al. (2005) Mol Pharmacol 68, 160-8.
- Dickerson, W.G. (1992) Dent Today 11, 54-5.
- Soroka, C.J. et al. (2001) Hepatology 33, 783-91.
- Nies, A.T. et al. (2001) Int J Cancer 94, 492-9.
- Ohishi, Y. et al. (2002) Clin Cancer Res 8, 3767-75.
- Plasschaert, S.L. et al. (2005) Clin Cancer Res 11, 8661-8.
- Benderra, Z. et al. (2005) Clin Cancer Res 11, 7764-72.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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