WB, IP
H M R Mk
Endogenous
80
Rabbit IgG
#Q9HCI7
55167
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Bovine, Dog, Pig, Horse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro210 of human MSL2 protein.
Background
Male-specific lethal 2-homolog (MSL2) is a component of the male-specific lethal (MSL) histone acetyltransferase complex, which contains MSL1, MSL2, MSL3, and MYST1. MYST1, also known as mammalian male absent on the first (MOF) and lysine acetyltransferase 8 (KAT8), is a member of the MYST (MOZ, YBF2, SAS2, and Tip60) family of histone acetyltransferases (1,2) and functions as the catalytic subunit of the MSL complex. The MSL complex is responsible for the majority of acetylation on histone H4 lysine 16 (3-5). In addition, as part of the MSL complex, MSL1 and MSL2 function as an E3 ubiquitin ligase to mono-ubiquitylate histone H2B on lysine 34 (6). The MSL complex plays a critical role in the regulation of transcription, DNA repair, autophagy, apoptosis, and embryonic stem cell pluripotency and differentiation (1,2,6,7). Loss of MSL activity leads to a global reduction in histone H4 lysine 16 acetylation, a common hallmark found in many human cancers. In particular, the reduction of MYST1 protein levels and histone H4 lysine 16 acetylation is associated with poor prognosis in breast, renal, colorectal, gastric, and ovarian cancers (1).
- Yang, Y. et al. (2014) Front Med 8, 79-83.
- Li, X. and Dou, Y. (2010) Epigenetics 5, 185-8.
- Dou, Y. et al. (2005) Cell 121, 873-85.
- Li, X. et al. (2009) Mol Cell 36, 290-301.
- Cai, Y. et al. (2010) J Biol Chem 285, 4268-72.
- Wu, L. et al. (2011) Mol Cell 43, 132-44.
- Füllgrabe, J. et al. (2013) Nature 500, 468-71.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
