WB
H M
Endogenous
50, 62
Rabbit IgG
#P50281
4323
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met293 of human MT1-MMP protein.
Background
The matrix metalloproteinase (MMP) family of proteases are a group of zinc-dependent enzymes that target extracellular proteins, including growth factors, cell surface receptors, adhesion molecules, and other proteases (1). Matrix metalloproteinases can be broadly categorized based on function and cellular localization, and include six distinct membrane-type (MT) metalloproteinases that share a transmembrane domain and short cytoplasmic tail (2). Membrane type-1 matrix metalloproteinase (MT1-MMP, MMP14) is involved in regulating development, angiogenesis, tissue remodeling, and tumor progression (3-6). MT1-MMP and other metalloproteinases promote tumor cell invasion by accumulating in specialized structures known as invadopodia, which remodel the ECM and allow tumor cells to breach the basement membrane (7). The abundance and presence of MT1-MMP at the cell surface is controlled by targeted endocytosis, which may be regulated by the MT1-MMP cytoplasmic domain (8). MT1-MMP protease activity can be further regulated through homodimer formation, autocatalytic processing, domain shedding, and the interaction with inhibitory proteins. Activation of the MT1-MMP proenzyme results from cleavage of full-length MT1-MMP by furin in the trans-Golgi network, which removes the inhibitory propeptide domain (9). At the cell surface, MT1-MMP can be found in a protein complex with the soluble metalloproteinase MMP2 and the MMP inhibitor TIMP2. MT1-MMP mediated cleavage and activation of MMP2 generate the active MMP2 collagenase, which plays important roles in ECM remodeling and tumor invasion (10). MT1-MMP interacts with a large number of substrates in addition to MMP2, including interstitial collagens, adhesive glycoproteins (i.e., laminin), and cell surface receptors (11).
- Kessenbrock, K. et al. (2010) Cell 141, 52-67.
- Nagase, H. et al. (2006) Cardiovasc Res 69, 562-73.
- Sounni, N.E. and Noel, A. (2008) Biochimie 87, 329-42.
- van Hinsbergh, V.W. and Koolwijk, P. (2008) Cardiovasc Res 78, 203-12.
- Rowe, R.G. and Weiss, S.J. (2008) Trends Cell Biol 18, 560-74.
- Tang, Y. et al. (2013) Dev Cell 25, 402-16.
- Poincloux, R. et al. (2009) J Cell Sci 122, 3015-24.
- Gingras, D. and Béliveau, R. (2010) Biochim Biophys Acta 1803, 142-50.
- Osenkowski, P. et al. (2004) J Cell Physiol 200, 2-10.
- Sato, H. and Takino, T. (2010) Cancer Sci 101, 843-7.
- Barbolina, M.V. and Stack, M.S. (2008) Semin Cell Dev Biol 19, 24-33.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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