WB, W-S
H
Endogenous
35-40, 65
Rabbit
#Q9GZU1
57192
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human mucolipin-1 protein. Antibodies are purified by peptide affinity chromatography.
Background
Mucolipin-1 (MCOLN1, TRPML1), a member of the transient receptor potential ion channel superfamily, is a lysosomal/late endosome Ca2+ efflux channel that is important in lysosomal biogenesis (1,2). Mutations in the mucolipin-1 gene are responsible for the human lysosomal storage disease mucolipidosis type IV (MLIV), an autosomal recessive neurodegenerative disease (3). Mucolipin-1 is synthesized as a 580-amino acid protein with six transmembrane domains but can undergo cleavage in the long luminal loop between the first two transmembrane domains (4,5). Mucolipin-1 activity has been linked to autophagy and lysosomal biogenesis. Calcium influx through mucolipin-1 activates CaMKKβ and AMPK, which activate the autophagy kinase ULK1 (6). Calcium influx is also associated with activation of the calcium-dependent phosphatase calcineurin which can trigger activation of TFEB, a key transcription factor regulating autophagy and lysosomal biogenesis (7,8). Mucolipin-1 activity is upregulated by cellular stress, including reactive oxygen species (ROS) (7). Phosphorylation of mucolipin-1 by mTOR results in reduced channel activity and autophagic flux (9). Loss of mucolipin-1 or pharmacological inhibition suppresses autophagy and can reduce tumor growth and metastasis in non-small cell lung cancer and triple-negative breast cancer, suggesting the use of mucolipin-1 antagonists for cancer therapy (10-12).
- Zeevi, D.A. et al. (2007) Biochim Biophys Acta 1772, 851-8.
- Cheng, X. et al. (2010) FEBS Lett 584, 2013-21.
- Mirabelli-Badenier, M. et al. (2015) Metab Brain Dis 30, 681-6.
- Kiselyov, K. et al. (2005) J Biol Chem 280, 43218-23.
- Miedel, M.T. et al. (2006) J Biol Chem 281, 12751-9.
- Scotto Rosato, A. et al. (2019) Nat Commun 10, 5630.
- Zhang, X. et al. (2016) Nat Commun 7, 12109.
- Zhang, X. et al. (2016) Autophagy 12, 1954-1955.
- Onyenwoke, R.U. et al. (2015) Biochem J 470, 331-42.
- Yin, C. et al. (2019) Cancer Manag Res 11, 8607-8617.
- Xu, M. et al. (2019) Cell Calcium 79, 80-88.
- Yang, Y. et al. (2020) Cells 9, 2682-2688.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting W-S: Simple Western™
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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