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18070
Mutant Ras Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Mutant Ras Antibody Sampler Kit #18070

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Mutant Ras Antibody Sampler Kit: Image 1

Western blot analysis of extracts from various cell lines using Ras (G12V Mutant Specific) (D2H12) Rabbit mAb (upper), Ras (D2C1) Rabbit mAb #8955 (middle), and β-Actin (D6A8) #8457 (lower).

Mutant Ras Antibody Sampler Kit: Image 2

Western blot analysis of extracts from various cell lines using Ras (G12D Mutant Specific) (D8H7) Rabbit mAb (upper), Ras (D2C1) Rabbit mAb #8955 (middle), and β-Actin (D6A8) #8457 (lower).

Mutant Ras Antibody Sampler Kit: Image 3

Western blot analysis of extracts from various cell lines using Ras (D2C1) Rabbit mAb.

Mutant Ras Antibody Sampler Kit: Image 4

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

To Purchase # 18070T
Product # Size Price
18070T
1 Kit  (3 x 20 µl) $ 261

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Ras (G12V Mutant Specific) (D2H12) Rabbit mAb 14412 20 µl
  • WB
H 21 Rabbit IgG
Ras (G12D Mutant Specific) (D8H7) Rabbit mAb 14429 20 µl
  • WB
H 21 Rabbit IgG
Ras (D2C1) Rabbit mAb 8955 20 µl
  • WB
H M R Mk 21 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Kit Usage Information

Protocols

Product Description

The Mutant Ras Antibody Sampler Kit provides an economical means of detecting common mutation in the Ras protein. The kit contains enough primary and secondary antibodies to perform at least two western blot experiments.

Specificity / Sensitivity

Each antibody in the Mutant Ras Antibody Sampler kit detects endogenous levels of its target protein. Ras (D2C1) Rabbit mAb recognizes endogenous levels of total Ras protein, specifically K-Ras and N-Ras. This antibody does not cross-react with H-Ras or R-Ras.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to G12V and G12D mutant sequences of human Ras protein. Ras (D2C1) Rabbit mAb was produced by immunizing rabbits with a recombinant protein specific to human K-Ras protein.

Background

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6). Additional oncogenic mutations of Ras have been observed at varying frequencies at codons 12, 13, and 61. The Gly12 to Val12 (G12V) mutation has been detected in a number of different cancers, including colorectal and thyroid cancer (7,8).

  1. Boguski, M.S. and McCormick, F. (1993) Nature 366, 643-54.
  2. Avruch, J. et al. (1994) Trends Biochem Sci 19, 279-83.
  3. Buday, L. and Downward, J. (1993) Cell 73, 611-20.
  4. Huang, D.C. et al. (1993) Mol Cell Biol 13, 2420-31.
  5. Bos, J.L. (1989) Cancer Res 49, 4682-9.
  6. Ma, J. and Karplus, M. (1997) J Mol Biol 274, 114-31.
  7. Prior, I.A. et al. (2012) Cancer Res 72, 2457-67.
  8. Winder, T. et al. (2009) Oncol Rep 21, 1283-7.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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