WB, IP, IHC-Bond, IHC-P
H
Endogenous
76
Rabbit IgG
#P20591
4599
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| IHC Leica Bond | 1:200 |
| Immunohistochemistry (Paraffin) | 1:160 |
Storage
For a carrier free (BSA and azide free) version of this product see product #62815.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu292 of human MX1 protein.
Background
MX1 (Myxovirus resistance protein 1/MxA) is an interferon-inducible antiviral protein that confers resistance to RNA viruses (1-4). MX1 has GTPase activity, and GTP-bound MX1 adopts a conformation that enables interaction with viral nucleocapsids (5-7). This interaction blocks transport of viral nucleocapsids to the nucleus, which prevents transcription of the viral genome (7,8). Structural studies suggest that the antiviral activity of MX1 involves the formation of MX1 oligomeric rings around viral nucleocapsids (9-12).
- Horisberger, M.A. and Hochkeppel, H.K. (1985) J Biol Chem 260, 1730-3.
- Staeheli, P. et al. (1985) J Biol Chem 260, 1821-5.
- Staeheli, P. and Haller, O. (1985) Mol Cell Biol 5, 2150-3.
- Staeheli, P. et al. (1986) Cell 44, 147-58.
- Nakayama, M. et al. (1991) J Biol Chem 266, 21404-8.
- Kochs, G. and Haller, O. (1999) J Biol Chem 274, 4370-6.
- Kochs, G. and Haller, O. (1999) Proc Natl Acad Sci U S A 96, 2082-6.
- Kochs, G. et al. (2002) Proc Natl Acad Sci U S A 99, 3153-8.
- Kochs, G. et al. (2002) J Biol Chem 277, 14172-6.
- Gao, S. et al. (2010) Nature 465, 502-6.
- Haller, O. et al. (2010) J Biol Chem 285, 28419-24.
- Gao, S. et al. (2011) Immunity 35, 514-25.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
