WB
M R
Endogenous
62
Rabbit
#Q96RE7
112939
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Mouse, Rat
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino acid sequence surrounding Ala321 of human NAC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
NAC1 or nuclear accumbens-1 is a nuclear factor that belongs to the POZ/BTB (Pox virus and zinc finger/bric-a-brac tramtrack broad complex) domain family. Also known as BTBD14B, it was originally identified in a unique neuronal forebrain structure responsible for reward motivation and addictive behaviors (1,2). NAC1 recruits HDAC3 and HDAC4 to transcriptionally repress gene expression in neuronal cells (3) and specifically co-represses other POZ/BTB proteins in the central nervous system (4). NAC1 is upregulated in several tumor types, including breast, renal cell, and hepatocellular carcinoma, as well as high grade ovarian serous carcinoma, where it has long been suspected as a chemoresistance gene (5,6). The chemoresistance mechanism reportedly occurs through NAC1 negative regulation of the GADD45 pathway (7). NAC1 has also been described as part of the extended transcriptional network in pluripotent cells that involves Oct-4, Sox2, Nanog, Sall1, KLF4 and Sall4 (8).
- Kalivas, P.W. et al. (1999) Synapse 33, 153-9.
- Mackler, S.A. et al. (2000) J Neurosci 20, 6210-7.
- Korutla, L. et al. (2005) J Neurochem 94, 786-93.
- Korutla, L. et al. (2009) Neurochem Int 54, 245-52.
- Nakayama, K. et al. (2006) Proc Natl Acad Sci USA 103, 18739-44.
- Yeasmin, S. et al. (2008) Clin Cancer Res 14, 1686-91.
- Jinawath, N. et al. (2009) Oncogene 28, 1941-8.
- Kim, J. et al. (2008) Cell 132, 1049-61.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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