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97174
NCAM1 (CD56) (123C3) Mouse mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

NCAM1 (CD56) (123C3) Mouse mAb (BSA and Azide Free) #97174

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  1. WB
  2. IHC
  3. IF
  4. F
Western Blotting Image 1: NCAM1 (CD56) (123C3) Mouse mAb (BSA and Azide Free)
Western blot analysis of extracts from human cerebellum using NCAM1 (CD56) (123C3) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: NCAM1 (CD56) (123C3) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human neuroblastoma, using NCAM1 (CD56) (123C3) Mouse mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: NCAM1 (CD56) (123C3) Mouse mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of SH-SY5Y cells (left) and HeLa cells (right) using NCAM1 (CD56) (123C3) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Data were generated using the standard formulation of this product.
Flow Cytometry Image 1: NCAM1 (CD56) (123C3) Mouse mAb (BSA and Azide Free)
Flow cytometric analysis of whole blood using NCAM1 (CD56) (123C3) Mouse mAb. Data were generated using the standard formulation of this product.
To Purchase # 97174
Cat. # Size Qty. Price
97174SF
100 µg
$ 776

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 120 to 220
Source/Isotype Mouse IgG1

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #3576. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #3576.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

NCAM (CD56) (123C3) Mouse mAb (BSA and Azide Free) detects endogenous levels of total CD56 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing BALB/c mice with a membrane preparation of a small cell lung carcinoma specimen.

Background

NCAM (neural cell adhesion molecule, CD56) is an adhesion glycoprotein with five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats. Structural diversity is introduced by alternative splicing resulting in different cytoplasmic domains (1). NCAM mediates neuronal attachment, neurite extension, and cell-cell interactions through homo and heterophilic interactions. PSA (polysialic acid) post-translationally modifies NCAM and increases the metastatic potential of small cell lung carcinoma, Wilms+ tumor, neuroblastoma, and rhabdomyosarcoma (2). CD56 is commonly used along with CD3 and CD16 to identify human natural killer (NK) cells (mouse NK cells do not express CD56) (3). Human NK cells are CD3-CD56+. The large subset with high CD16 expression are mature cytotoxic NK cells, while those with low CD16 expression are immature precursors and cytokine producers (4,5).
  1. Cunningham, B. A. et al. (1987) Science 236, 799-806.
  2. Seidenfaden, R. et al. (2003) Mol. Cell. Biol. 23, 5908-5918.
  3. Hayakawa, Y. et al. (2006) Immunol Rev 214, 47-55.
  4. Robertson, M.J. and Ritz, J. (1990) Blood 76, 2421-38.
  5. Poli, A. et al. (2009) Immunology 126, 458-65.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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