IP, FC-L
H
Endogenous
70-80
Rabbit IgG
#Q92692
5819
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunoprecipitation | 1:100 |
| Flow Cytometry (Live) | 1:100 - 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Nectin-2/CD112 protein.
Background
Nectin-2, also known as CD112 and poliovirus receptor-related 2 (PVRL2), is a single-pass type I membrane glycoprotein ubiquitously expressed on various human tissues (1). It is a calcium independent cell adhesion molecule known to interact with several cell surface receptors, including DNAM-1 (CD226), LFA-1 (CD11a), Nectin-3 (CD113), TIGIT (VSTM3), and PVRIG (CD112R) (2-7). It is one of the major constituents of adherens junctions, and therefore plays a central role in a number of cellular processes, including adhesion, migration, and proliferation (2-8). Within the immune system, Nectin-2 modulates immune cell signaling. Upon interaction with DNAM-1 expressed on T and NK cells, Nectin-2 stimulates proliferation and cytokine production (4). Upon interaction with PVRIG, Nectin-2 inhibits proliferation (7). Thus, Nectin-2 can be either a co-stimulator or a co-inhibitor of immune cell function depending on competitive receptor interactions. Nectin-2 also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses (8,9). Alternate transcriptional splice variants, encoding different isoforms, have been characterized (10-12).
- Eberlé, F. et al. (1995) Gene 159, 267-72.
- Lopez, M. et al. (1998) Blood 92, 4602-11.
- Satoh-Horikawa, K. et al. (2000) J Biol Chem 275, 10291-9.
- Bottino, C. et al. (2003) J Exp Med 198, 557-67.
- Reymond, N. et al. (2004) J Exp Med 199, 1331-41.
- Deuss, F.A. et al. (2017) J Biol Chem 292, 11413-22.
- Zhu, Y. et al. (2016) J Exp Med 213, 167-76.
- Devilard, E. et al. (2013) PLoS One 8, e77424.
- Martinez, W.M. and Spear, P.G. (2001) J Virol 75, 11185-95.
- Warner, M.S. et al. (1998) Virology 246, 179-89.
- Ota, T. et al. (2004) Nat Genet 36, 40-5.
- Gerhard, D.S. et al. (2004) Genome Res 14, 2121-7.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IP: Immunoprecipitation FC-L: Flow Cytometry (Live)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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