| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Neutrophil Elastase (E9C9L) XP® Rabbit mAb | 89241 | 20 µl | 30 kDa | Rabbit IgG |
| Myeloperoxidase (E1E7I) XP® Rabbit mAb | 14569 | 20 µl | 60, 80-90 kDa | Rabbit IgG |
| Histone H3 (D1H2) XP® Rabbit mAb | 4499 | 20 µl | 17 kDa | Rabbit IgG |
| Cathepsin G (E3N3O) Rabbit mAb | 63665 | 20 µl | 29 kDa | Rabbit IgG |
| Citrullinated Histone H3 (Arg17) (E4O3F) Rabbit mAb | 97272 | 20 µl | 17 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The NETosis Antibody Sampler Kit provides an economical means of detecting proteins involved in NETosis. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Storage
Background
NETosis is a unique form of regulated cell death that is characterized by membrane rupture and the extrusion of chromatin, histones, and granular and cytoplasmic components into a web-like structure called neutrophil extracellular traps (NETs) (reviewed in 1). NETosis has been associated with host defense to pathogens as well as a number of disease states, including autoimmune diseases, thrombosis, cardiovascular diseases, and tumor progression. NETosis was identified as a response to bacterial infection and can be activated by lipopolysaccharide (LPS) as well as inflammatory pathway activators like phorbol-12-myristate-13-acetate (PMA) (2). It can occur via multiple pathways, but several key players have emerged. The calcium-dependent enzyme protein-arginine deiminase 4 (PAD4) catalyzes hypercitrullination of histones that contributes to chromatin decondensation (3,4). In addition, activation of proteases, including neutrophil elastase (ELANE), myeloperoxidase (MPO), and Cathepsin G, leads to impairment of cytoskeletal structures and degradation of histones during NETosis (5,6).
- Thiam, H.R. et al. (2020) Annu Rev Cell Dev Biol 36, 191-218.
- Brinkmann, V. et al. (2004) Science 303, 1532-5.
- Li, P. et al. (2010) J Exp Med 207, 1853-62.
- Wong, S.L. and Wagner, D.D. (2018) FASEB J, fj201800691R.
- Papayannopoulos, V. et al. (2010) J Cell Biol 191, 677-91.
- Metzler, K.D. et al. (2014) Cell Rep 8, 883-96.
Background References
Trademarks and Patents
Limited Uses
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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
