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9781
Neurofilament Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Neurofilament Antibody Sampler Kit #9781

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Neurofilament Antibody Sampler Kit: Image 1

Immunohistochemical analysis of paraffin-embedded human brain, using Neurofilament-M (RMO 14.9) Mouse mAb.

Neurofilament Antibody Sampler Kit: Image 2

Confocal immunofluorescent image of mouse cerebellum labeled with Neurofilament-L (DA2) Mouse mAb (green) and CREB (48H2) Rabbit mAb #9197 (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

Neurofilament Antibody Sampler Kit: Image 3

Confocal immunofluorescent analysis of normal rat cerebellum using Neurofilament-L (C28E10) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Neurofilament Antibody Sampler Kit: Image 4

Confocal immunofluorescent image of mouse cerebellum labeled with Neurofilament-H (RMdO 20) Mouse mAb (green) and Calbindin Antibody #2136 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Neurofilament Antibody Sampler Kit: Image 5

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Neurofilament Antibody Sampler Kit: Image 6

Western blot analysis of extracts from rat brain, untreated or treated with lambda phosphatase, using Neurofilament-M (RMO 14.9) Mouse mAb.

Neurofilament Antibody Sampler Kit: Image 7

Immunohistochemical analysis of paraffin-embedded human brain using Neurofilament-L (DA2) Mouse mAb.

Neurofilament Antibody Sampler Kit: Image 8

Immunohistochemical analysis of paraffin-embedded mouse brain using Neurofilament-L (C28E10) Rabbit mAb.

Neurofilament Antibody Sampler Kit: Image 9

Immunohistochemical analysis of paraffin-embedded human brain, using Neurofilament-H (RMdO 20) Mouse mAb.

Neurofilament Antibody Sampler Kit: Image 10

Immunohistochemical analysis of paraffin-embedded human medulloblastoma, using Neurofilament-L (DA2) Mouse mAb.

Neurofilament Antibody Sampler Kit: Image 11

Immunohistochemical analysis of paraffin-embedded human brain using Neurofilament-L (C28E10) Rabbit mAb in the presence of control peptide (left) or Neurofilament-L blocking peptide #1005 (right).

Neurofilament Antibody Sampler Kit: Image 12

Western blot analysis of extracts from mouse brain, rat brain or CAD cells, using Neurofilament-H (RMdO 20) Mouse mAb.

Neurofilament Antibody Sampler Kit: Image 13

Western blot analysis of extracts from rat brain, mouse brain, U87-MG cells, C6 cells and human cerebellum, using Neurofilament-L (DA2) Mouse mAb.

Neurofilament Antibody Sampler Kit: Image 14

Western blot analysis of extracts from mouse brain, HeLa cells and rat brain, using Neurofilament-L (C28E10) Rabbit mAb.

To Purchase # 9781T
Product # Size Price
9781T
1 Kit  (4 x 20 µl) $ 327

Product Description

The Neurofilament Antibody Sampler Kit provides an economical means of evaluating neurofilaments. The kit contains enough primary and secondary antibodies to perform two western blot experiments per primary antibody.

Specificity / Sensitivity

Neurofilament-L (DA2) Mouse mAb and Neurofilament-L (C28E10) Rabbit mAb detect endogenous levels of total Neurofilament-L protein. Neurofilament-M (RMO 14.9) Mouse mAb detects endogenous levels of total Neurofilament-M protein. Neurofilament-H (RMdO 20) Mouse mAb detects endogenous levels of total Neurofilament-H protein. Neurofilament-H (RMdO 20) Mouse mAb has been reported to detect NFM and NFH in human samples but only NFH in mouse, rat or bovine samples (Lee, V.M. et al., 1988).

Source / Purification

Monoclonal antibodies are produced by immunizing animals with purified and enzymatically dephosphorylated pig neurofilament, light chain (Neurofilament-L (DA2)), a synthetic peptide surrounding Glu450 of human Neurofilament-L (Neurofilament-L (C28E10)), rat neurofilament medium chain

(Neurofilament-M (RMO 14.9)), or rat neurofilament (Neurofilament-H (RMdO 20)).

#2836 Monoclonal antibody is produced by immunizing animals with rat neurofilament.

#2835 Monoclonal antibody is produced by immunizing animals with purified and enzymatically dephosphorylated pig neurofilament, light chain.

#2837 Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Glu450 of human Neurofilament-L.

#2838 Monoclonal antibody is produced by immunizing animals with rat neurofilament, medium chain.

Background

The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).

  1. Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-55.
  2. Cohlberg, J.A. et al. (1995) J Biol Chem 270, 9334-9.
  3. Hisanaga, S. et al. (1994) Mol Biol Cell 5, 161-72.
  4. Sihag, R.K. et al. (1999) J Neurochem 72, 491-9.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.