WB, IF-IC
H M R
Endogenous
50-75
Rabbit IgG
#O00712
4781
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:3200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro404 of human NFI-B protein.
Background
NFI-B belongs to the nuclear factor I (NFI) family of site-specific transcription factors that regulate viral DNA replication and expression of various genes (1,2). The NFI family is composed of four members in vertebrates: NFI-A, NFI-B, NFI-C, and NFI-X, all of which are critical in the development of multiple organ systems in mice and humans (3). Research studies have demonstrated that NFI-B plays a crucial role in different aspects of tumor development, acting as an oncogene or tumor suppressor in different types of tumors and participating in tumor-associated fusion gene formation (4-7). Moreover, NFI-B has been shown to promote epithelial-mesenchymal transition and metastatic ability of cancer cells through a number of mechanisms, including upregulating SNAI1 expression in colorectal cancer (8), stabilizing chromatin accessibility in small cell lung cancer (9), and modulating the ZEB1 axis in melanoma (10).
- Gronostajski, R.M. (2000) Gene 249, 31-45.
- Liang, B. et al. (2012) J Virol 86, 3264-75.
- Steele-Perkins, G. et al. (2003) Mol Cell Biol 23, 1075-84.
- Stringer, B.W. et al. (2016) Oncotarget 7, 29306-20.
- Moon, H.G. et al. (2011) Mol Oncol 5, 538-44.
- Wang, N. et al. (2021) PeerJ 9, e10848.
- Wagner, V.P. et al. (2022) Crit Rev Oncol Hematol 176, 103745.
- Liu, Z. et al. (2019) Cancer Sci 110, 86-98.
- Denny, S.K. et al. (2016) Cell 166, 328-342.
- Cheng, R. et al. (2021) Oncol Lett 21, 81.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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