WB, W-S
H
Endogenous
125
Rabbit IgG
#Q99558
9020
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NIK protein.
Background
Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: Rel-A, c-Rel, Rel-B, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. The p50 and p52 products form dimeric complexes with Rel proteins, which are then able to bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by its inhibitory proteins, the IκBs (3-5). NF-κB-activating agents can induce the phosphorylation of IκBs, which targets them for rapid degradation through a ubiquitin-proteasome pathway, releasing NF-κB to enter the nucleus and regulate gene expression (6-8). Processing of NF-κB2 is regulated by IKK1 (IKKα), which triggers the phosphorylation and processing to p52, which can then undergo nuclear translocation (9-11).
Activation of NF-κB can be controlled by NF-kB-inducing kinase (NIK), a member of the MAP3K family that was originally identified as a TRAF2-interacting protein and thereby coupled to receptor activation (12). NIK forms a complex with and phosphorylates IKK1 and IKK2, subsequently leading to the phosphorylation of IκB and translocation of NF-κB to the nucleus (13-15).
- Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
- Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
- Haskill, S. et al. (1991) Cell 65, 1281-9.
- Thompson, J.E. et al. (1995) Cell 80, 573-82.
- Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
- Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
- Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
- Chen, Z.J. et al. (1996) Cell 84, 853-62.
- Senftleben, U. et al. (2001) Science 293, 1495-9.
- Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
- Xiao, G. et al. (2001) Mol Cell 7, 401-9.
- Malinin, N.L. et al. (1997) Nature 385, 540-4.
- Régnier, C.H. et al. (1997) Cell 90, 373-83.
- Woronicz, J.D. et al. (1997) Science 278, 866-9.
- Ling, L. et al. (1998) Proc Natl Acad Sci USA 95, 3792-7.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting W-S: Simple Western™
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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