WB, IP, IHC-P
H
Endogenous
7-25
Rabbit IgG
#Q16617
4818
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly161 of human NKG7 protein.
Background
Natural killer cell granule protein 7 (NKG7, also known as GMP-17 and GIG-I) is a novel type 1 integral membrane protein expressed at high levels in lymphocytes, including natural killer (NK) cells, γδ T cells, a subset of αβ T cells, lymphokine-activated killer (LAK) cells (1), and activated T regulatory cells (2). NKG7 expression can be induced in neutrophils following GM-CSF treatment (3). NKG7 protein is localized to the membranes of cytotoxic granules. Following target cell-induced NK cell degranulation, NKG7 translocates from granules to the plasma membrane (4). Studies have also shown that NKG7 is significantly upregulated in allograft rejection patients (5-8).
- Turman, M.A. et al. (1993) Hum Immunol 36, 34-40.
- Sugimoto, N. et al. (2006) Int Immunol 18, 1197-209.
- Shimane, M. et al. (1994) Biochem Biophys Res Commun 199, 26-32.
- Medley, Q.G. et al. (1996) Proc Natl Acad Sci U S A 93, 685-9.
- Sacreas, A. et al. (2018) PLoS One 13, e0205107.
- Sigdel, T.K. et al. (2015) PLoS One 10, e0138133.
- Ashokkumar, C. et al. (2011) Am J Pathol 179, 1929-38.
- Meehan, S.M. et al. (1997) Lab Invest 76, 639-49.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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