WB, IHC-P
H
Endogenous
30
Rabbit IgG
#Q99801
4824
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:250 |
Storage
For a carrier free (BSA and azide free) version of this product see product #32253.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NKX3.1 protein. The epitope is near the amino terminus, in a region that is 100% conserved between isoforms 1 and 5 of human NKX3.1.
Background
NKX3.1 is a homeobox transcription factor that in mammals plays a defining role in embryonic prostate morphogenesis. The expression of mammalian NKX3.1 is androgen-dependent, restricted primarily to developing and mature prostate epithelium, and is frequently reduced or lost in prostate cancer (1-3). The human NKX3.1 gene is located on chromsome 8p21.2, within a region that shows loss of heterozygosity (LOH) in >50% of prostate cancer cases (2). Allelic loss at the NKX3.1 locus is also common in high grade Prostate Intraepithelial Neoplasia (PIN), thought to be a putative precursor lesion to invasive prostate adenocarcinomas, suggesting that LOH at the NKX3.1 locus is a critical early step in prostate cancer development (4). Notably, the remaining NKX3.1 allele is intact in the majority of LOH cases, leading to the suggestion that NKX3.1 functions as a haploinsufficient tumor suppressor (4-6). Due to its highly restricted expression in prostate epithelial cells, NKX3.1 has been suggested as a diagnostic marker of prostate carcinoma (7), and may have additional utility as a biomarker of metastatic lesions originating in the prostate (8).
- Bhatia-Gaur, R. et al. (1999) Genes Dev 13, 966-77.
- He, W.W. et al. (1997) Genomics 43, 69-77.
- Bowen, C. et al. (2000) Cancer Res 60, 6111-5.
- Magee, J.A. et al. (2003) Cancer Cell 3, 273-83.
- Voeller, H.J. et al. (1997) Cancer Res 57, 4455-9.
- Bethel, C.R. et al. (2006) Cancer Res 66, 10683-90.
- Epstein, J.I. et al. (2014) Am J Surg Pathol 38, e6-e19.
- Conner, J.R. and Hornick, J.L. (2015) Adv Anat Pathol 22, 149-67.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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