Cat. # | Size | Qty. | Price |
---|---|---|---|
20779S | 100 µl |
|
REACTIVITY | H Mk |
SENSITIVITY | Endogenous |
MW (kDa) | 78 |
Source/Isotype | Rabbit IgG |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 263
Human, Monkey
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NO66 protein.
Nucleolor protein 66 (NO66), also known as Myc-associated protein with a jumonji C (JmjC) domain (MAPJD), or ribosomal oxygenase 1, belongs to a large family of JmjC-domain-containing oxygenase proteins. NO66 exhibits both ribosomal histidine hydroxylase and histone demethylase activities, and plays a key role in regulation of gene transcription, RNA processing, and translation. NO66-mediated hydroxylation of ribosomal protein L8 (Rpl8) may play a role in regulation of protein synthesis (1). NO66 also functions to repress transcription by demethylating histone H3 lys4 and lys36, two histone marks that are important for transcriptional activation (2). The interaction of NO66 with the transcription factor osterix (OSX) regulates osteoblast differentiation and bone formation through repression of OSX target genes (3,4). In embryonic stem cells, the PHF19 protein recruits NO66 along with polycomb repressor complex 2 (PRC2) to differentiation-specific target genes to repress transcription through demethylation of histone H3 lys36 and methylation of histone H3 lys27, the latter mark being associated with transcriptional repression (2). NO66 is overexpressed in non-small cell lung cancer and colorectal cancer, and is associated with poor prognosis (5,6).
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.