| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Brg1 (D1Q7F) Rabbit mAb | 49360 | 20 µl | 220 kDa | Rabbit IgG |
| BRD9 (E9R2I) Rabbit mAb | 58906 | 20 µl | 80 kDa | Rabbit IgG |
| GLTSCR1 (E6I3A) Rabbit mAb | 45441 | 20 µl | 205 kDa | Rabbit IgG |
| GLTSCR1L (E9K6F) Rabbit mAb | 92805 | 20 µl | 140 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
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Description
The Non-Canonical BAF Complex Antibody Sampler Kit provides an economical means of detecting non-canonical BAF complex members using antibodies. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Storage
Background
ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1, to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1,6-9).
GLTSCR1 and its paralog GLTSCR1L, along with BRD9, have been identified as unique subunits of the non-canonical BAF (ncBAF) or GBAF complex. This complex contains either GLTSCR1 or GLTSCR1L instead of an ARID subunit, while also lacking the BAF45, BAF47, and BAF57 subunits. GBAF maps to regions distinct from the PBAF and canonical BAF complexes and has also been shown to be a synthetic lethal target for BAF driven cancers such as synovial sarcomas and rhabdoid tumors (10-12).
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- Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
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- Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
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- Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
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- Alpsoy, A. and Dykhuizen, E.C. (2018) J Biol Chem 293, 3892-3903.
- Mashtalir, N. et al. (2018) Cell 175, 1272-1288.e20.
- Michel, B.C. et al. (2018) Nat Cell Biol 20, 1410-1420.
Background References
Trademarks and Patents
Limited Uses
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