Revision 2
#3640
Store at -20C
Notch Isoform Antibody Sampler Kit
1 Kit
(4 x 20 microliters)
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Cleaved Notch1 (Val1744) (D3B8) Rabbit Monoclonal Antibody | 4147 | 20 µl | 110 kDa | Rabbit IgG |
| Notch1 (D1E11) Rabbit Monoclonal Antibody | 3608 | 20 µl | 120, 300 kDa | Rabbit IgG |
| Notch3 (D11B8) Rabbit Monoclonal Antibody | 5276 | 20 µl | 90, 270 kDa | Rabbit IgG |
| Notch2 (D76A6) Rabbit Monoclonal Antibody | 5732 | 20 µl | 110, 300 kDa | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
Storage
Background
Constitutively activated Notch1 signaling is associated with the majority of cases of T cell acute lymphoblastic leukemia (T-ALL). The activation is either due to mutations in Notch1 itself or in the components of ubiquitin ligase complex, namely FBW7 (5-6). Notch2 is a member of the Notch family and mutation in Notch2 is associated with Alagille syndrome (7). Notch3 is a member of the Notch family and is processed similar to Notch1 (8). It is expressed primarily in arterial smooth muscle cells (SMC). Mutations altering the number of cysteine residues in the notch3 extracellular region are associated with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), a hereditary angiopathy leading to strokes and dementia in adults (9-11). Recent studies indicate that Notch3 is overexpressed in many types of cancer (12-14).
Background References
- Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
- Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
- Schroeter, E.H. et al. (1998) Nature 393, 382-6.
- Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
- Weng, A.P. et al. (2004) Science 306, 269-71.
- Thompson, B.J. et al. (2007) J Exp Med 204, 1825-35.
- McDaniell, R. et al. (2006) Am J Hum Genet 79, 169-73.
- Baron, M. (2003) Semin Cell Dev Biol 14, 113-9.
- Kalimo, H. et al. (2002) Brain Pathol 12, 371-84.
- Karlström, H. et al. (2002) Proc Natl Acad Sci U S A 99, 17119-24.
- Monet, M. et al. (2007) Hum Mol Genet 16, 982-92.
- Park, J.T. et al. (2006) Cancer Res 66, 6312-8.
- Gramantieri, L. et al. (2007) Liver Int 27, 997-1007.
- Yamaguchi, N. et al. (2008) Cancer Res 68, 1881-8.
Trademarks and Patents
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Limited Uses
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