WB, IP
H M R Mk
Endogenous
58
Rabbit IgG
#P51513
4857
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala36 of human NOVA1 protein.
Background
Neuro-oncological ventral antigen (NOVA) 1 and 2 are neuronal RNA-binding proteins (RBPs) that bind YCAY motifs to regulate alternative splicing (1-3). Both proteins have been shown to be targets in paraneoplastic opsoclonus myoclonus ataxia, a neuronal autoimmune disease (1,3). NOVA proteins rely on their KH3 domain to bind RNA and are reciprocally expressed in various parts of the brain (4). NOVA1 has been shown to be essential for neuronal viability, as it controls alternative splicing in GlyRalpha2 and GABA(A) receptor pre-mRNAs (5). NOVA1 is also capable of auto-regulation as it binds to its own exon 4, which contains a phosphorylated protein domain (6). NOVA1/2 double knockout mice are paralyzed due to lack of alternatively spliced agrin Z(+), which helps synapse formation at neuromuscular junctions (7). NOVA2 has been shown to regulate circular RNA biogenesis in the developing brain (8). NOVA1 has been shown to be highly expressed in many different cancer types, and can promote epithelial-mesenchymal transition by binding β-catenin mRNA (9,10).
- Buckanovich, R.J. et al. (1996) J Neurosci 16, 1114-22.
- Buckanovich, R.J. and Darnell, R.B. (1997) Mol Cell Biol 17, 3194-201.
- Yang, Y.Y. et al. (1998) Proc Natl Acad Sci U S A 95, 13254-9.
- Lewis, H.A. et al. (1999) Structure 7, 191-203.
- Dredge, B.K. et al. (2005) EMBO J 24, 1608-20.
- Ruggiu, M. et al. (2009) Proc Natl Acad Sci U S A 106, 3513-8.
- Cao, Y. et al. (2021) Mol Cell Biochem 476, 279-292.
- Knupp, D. et al. (2021) Nucleic Acids Res 49, 6849-6862.
- Liu, M. et al. (2020) Transl Cancer Res 9, 4373-4382.
- Tang, S. et al. (2020) RNA Biol 17, 881-891.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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