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12784
Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit #12784

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To Purchase # 12784

Product Description

The Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit provides an economical means to evaluate relevant nuclear proteins. This kit contains enough primary antibody to perform at least four western blots per primary antibody.

Specificity / Sensitivity

Each antibody in the Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit recognizes total endogenous levels of the specific target protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human ESET protein, residues surrounding Thr298 of human fibrillarin, the carboxy terminus of the human histone H3 protein, a recombinant fragment of human lamin A protein, residues near the amino-terminus of human LSD1 protein, or residues surrounding Pro671 of human NUP98. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human histone H2A.Z. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

The Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit contains a variety of antibodies directed against established nuclear proteins (1). Histone H3 and histone H2A.Z are histone family members and components of nucleosomes, the primary building block of chromatin made up of DNA wound around eight core histone proteins. The amino-terminal tails of core histones undergo various post-translational modifications and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (2). ESET histone methyltransferase (3) and LSD1 histone demethylase (4) are both regulators of histone methylation and are chromatin-associated. Both NUP98 (5) and lamins (6) are located within the nuclear envelope (also known as the nuclear membrane). NUP98 is a component of the nuclear pore complex. Lamin A and lamin C are fibrous proteins contributing to nuclear structural and transcriptional regulation. Finally, fibrillarin (7) is located in fibrillar regions and Cajal bodies of nucleoli, where it functions to regulate RNA transcription and pre-rRNA processing.

  1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
  2. Jin, J. et al. (2005) Trends Biochem Sci 30, 680-7.
  3. Yang, L. et al. (2002) Oncogene 21, 148-152.
  4. Shi, Y. et al. (2004) Cell 119, 941-953.
  5. Fontoura, B.M. et al. (1999) J Cell Biol 144, 1097-112.
  6. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
  7. Tollervey, D. et al. (1993) Cell 72, 443-57.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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