The Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit provides an economical means to evaluate relevant nuclear proteins. This kit contains enough primary antibody to perform at least four western blots per primary antibody.
Each antibody in the Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit recognizes total endogenous levels of the specific target protein.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human ESET protein, residues surrounding Thr298 of human fibrillarin, the carboxy terminus of the human histone H3 protein, a recombinant fragment of human lamin A protein, residues near the amino-terminus of human LSD1 protein, or residues surrounding Pro671 of human NUP98. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human histone H2A.Z. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
The Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit contains a variety of antibodies directed against established nuclear proteins (1). Histone H3 and histone H2A.Z are histone family members and components of nucleosomes, the primary building block of chromatin made up of DNA wound around eight core histone proteins. The amino-terminal tails of core histones undergo various post-translational modifications and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (2). ESET histone methyltransferase (3) and LSD1 histone demethylase (4) are both regulators of histone methylation and are chromatin-associated. Both NUP98 (5) and lamins (6) are located within the nuclear envelope (also known as the nuclear membrane). NUP98 is a component of the nuclear pore complex. Lamin A and lamin C are fibrous proteins contributing to nuclear structural and transcriptional regulation. Finally, fibrillarin (7) is located in fibrillar regions and Cajal bodies of nucleoli, where it functions to regulate RNA transcription and pre-rRNA processing.
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